ReferenceID 5591
Leonurine Protects Bone Mesenchymal Stem Cells from Oxidative Stress by Activating Mitophagy through PI3K/Akt/mTOR Pathway
Cells
Osteoporosis bears an imbalance between bone formation and resorption, which is strongly related to oxidative stress. The function of leonurine on bone marrow-derived mesenchymal stem cells (BMSCs) under oxidative stress
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Record Fields
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- Reference Id
- 5591
- Evidence Id
- 22181
- Core Evidence Id
- 22181
- Source Reference Id
- 4394
- Herb2 Reference Id
- HBREF005191
- Subject Paper Key
- HBIN032907_35681421
- Pubmed Id
- 35681421
- Doi
- 10.3390/cells11111724
- Paper Title
- Leonurine Protects Bone Mesenchymal Stem Cells from Oxidative Stress by Activating Mitophagy through PI3K/Akt/mTOR Pathway
- Paper Abstract
- Osteoporosis bears an imbalance between bone formation and resorption, which is strongly related to oxidative stress. The function of leonurine on bone marrow-derived mesenchymal stem cells (BMSCs) under oxidative stress is still unclear. Therefore, this study was aimed at identifying the protective effect of leonurine on H 2 O 2 stimulated rat BMSCs. We found that leonurine can alleviate cell apoptosis and promote the differentiation ability of rat BMSCs induced by oxidative stress at an appropriate concentration at 10 μM. Meanwhile, the intracellular ROS level and the level of the COX2 and NOX4 mRNA decreased after leonurine treatment in vitro. The ATP level and mitochondrial membrane potential were upregulated after leonurine treatment. The protein level of PINK1 and Parkin showed the same trend. The mitophage in rat BMSCs blocked by 3-MA was partially rescued by leonurine. Bioinformatics analysis and leonurine-protein coupling provides a strong direct combination between leonurine and the PI3K protein at the position of Asp841, Glu880, Val882. In conclusion, leonurine protects the proliferation and differentiation of BMSCs from oxidative stress by activating mitophagy, which depends on the PI3K/Akt/mTOR pathway. The results showed that leonurine may have potential usage in osteoporosis and bone defect repair in osteoporosis patients.
- Journal
- Cells
- Publish Year
- 2022
- Experiment Subject
- rat; patient; bone marrow-derived mesenchymal stem cells; h 2 o 2 stimulated rat bmscs
- Experiment Type
- Animal & Cell Experiment
- Phenotype Related
- Osteoporosis; Bone Defect
- Paper Title Cn
- Paper Title En
- Leonurine Protects Bone Mesenchymal Stem Cells from Oxidative Stress by Activating Mitophagy through PI3K/Akt/mTOR Pathway
- Bilingual Status
- semi_complete