ReferenceID 5581

Combined effects of vitamin C and cold atmospheric plasma-conditioned media against glioblastoma via hydrogen peroxide

Free Radic Biol Med

Glioblastoma is the most lethal intracranial malignant tumor, for which the five-year overall survival rate is approximately 5%. Here we explored the therapeutic combination of vitamin C and plasma-conditioned medium on

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Reference Id
5581
Evidence Id
22171
Core Evidence Id
22171
Source Reference Id
4381
Herb2 Reference Id
HBREF005178
Subject Paper Key
HBIN032689_36436726
Pubmed Id
36436726
Doi
10.1016/j.freeradbiomed.2022.11.028
Paper Title
Combined effects of vitamin C and cold atmospheric plasma-conditioned media against glioblastoma via hydrogen peroxide
Paper Abstract
Glioblastoma is the most lethal intracranial malignant tumor, for which the five-year overall survival rate is approximately 5%. Here we explored the therapeutic combination of vitamin C and plasma-conditioned medium on glioblastoma cells in culture and as subcutaneous or intracranial xenografts in mice. The combination treatment reduced cell viability and proliferation while promoting apoptosis, and the effects were significantly stronger than with either treatment on its own. Similar results were obtained in the two xenograft models. Vitamin C appeared to upregulate aquaporin-3 and enhance the uptake of extracellular H 2 O 2 , while the combination treatment increased intracellular levels of reactive oxygen species including H 2 O 2 and activated the JNK signaling pathway. The cytotoxic effects of the combination treatment were partially reversed by the specific JNK signaling inhibitor SP600125. Our results suggest that the combination of vitamin C and plasma-conditioned medium has therapeutic potential against glioblastoma, and they provide mechanistic insights that may help investigate this and other potential therapies in greater depth.
Journal
Free Radic Biol Med
Publish Year
2022
Experiment Subject
mouse
Experiment Type
Animal & Cell Experiment
Phenotype Related
Glioblastoma; Intracranial Malignant Tumor
Paper Title Cn
Paper Title En
Combined effects of vitamin C and cold atmospheric plasma-conditioned media against glioblastoma via hydrogen peroxide
Bilingual Status
semi_complete