ReferenceID 5537
Juglone prevents human platelet aggregation through inhibiting Akt and protein disulfide isomerase
Phytomedicine
BACKGROUND/PURPOSE: Juglone, a natural compound widely found in Juglandaceae plants, has been suggested as a potential drug candidate for treating cancer, inflammation, and diabetic vascular complications. In the present
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Record Fields
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- Reference Id
- 5537
- Evidence Id
- 22127
- Core Evidence Id
- 22127
- Source Reference Id
- 4305
- Herb2 Reference Id
- HBREF005102
- Subject Paper Key
- HBIN031581_33387969
- Pubmed Id
- 33387969
- Doi
- 10.1016/j.phymed.2020.153449
- Paper Title
- Juglone prevents human platelet aggregation through inhibiting Akt and protein disulfide isomerase
- Paper Abstract
- BACKGROUND/PURPOSE: Juglone, a natural compound widely found in Juglandaceae plants, has been suggested as a potential drug candidate for treating cancer, inflammation, and diabetic vascular complications. In the present study, the antiplatelet effect and underlying mechanisms of juglone were investigated for the first time. STUDY DESIGN/METHODS: Human platelet aggregation and activation were measured by turbidimetric aggregometry, flow cytometry, and Western blotting. In vitro antithrombotic activity of juglone was assessed using collagen-coated flow chambers under whole-blood flow conditions. The effect of juglone on protein disulfide isomerase (PDI) activity was determined by the dieosin glutathione disulfide assay. RESULTS: Juglone (1 - 5 muM) inhibited platelet aggregation and glycoprotein (GP) IIb/IIIa activation caused by various agonists. In a whole blood flow chamber system, juglone reduced thrombus formation on collagen-coated surfaces under arterial shear rates. Juglone abolished intracellular Ca2+ elevation and protein kinase C activation caused by collagen, but had no significant effect on that induced by G protein-coupled receptor agonists. In contrast, Akt activation caused by various agonists were inhibited in juglone-treated platelets. Additionally, juglone showed inhibitory effects on both recombinant human PDI and platelet surface PDI at concentrations similar to those needed to prevent platelet aggregation. CONCLUSION: Juglone exhibits potent in vitro antiplatelet and antithrombotic effects that are associated with inhibition of Akt activation and platelet surface PDI activity.
- Journal
- Phytomedicine
- Publish Year
- 2021
- Experiment Subject
- human; juglone-treated platelets
- Experiment Type
- Cell Experiment
- Phenotype Related
- Diabetic Vascular Complications; Cancer; Inflammation
- Paper Title Cn
- Paper Title En
- Juglone prevents human platelet aggregation through inhibiting Akt and protein disulfide isomerase
- Bilingual Status
- semi_complete