ReferenceID 5521
Protective effects of isorhamnetin on pulmonary arterial hypertension: in vivo and in vitro studies
Phytother Res
Pulmonary arterial hypertension (PAH) is a malignant disease with high mortality and closely involves the bone morphogenetic protein (BMP) pathway. Mutations in BMPR2 caused proliferation of pulmonary artery smooth muscl
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Record Fields
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- Reference Id
- 5521
- Evidence Id
- 22111
- Core Evidence Id
- 22111
- Source Reference Id
- 4281
- Herb2 Reference Id
- HBREF005078
- Subject Paper Key
- HBIN031114_32452118
- Pubmed Id
- 32452118
- Doi
- 10.1002/ptr.6714
- Paper Title
- Protective effects of isorhamnetin on pulmonary arterial hypertension: in vivo and in vitro studies
- Paper Abstract
- Pulmonary arterial hypertension (PAH) is a malignant disease with high mortality and closely involves the bone morphogenetic protein (BMP) pathway. Mutations in BMPR2 caused proliferation of pulmonary artery smooth muscle cells (PASMCs) leading to PAH. Isorhamnetin, one of the main naturally occurring flavonoids extracted from Hippophae rhamnoides L, shows antiinflammatory and anti-proliferative properties. Nevertheless, the effects of isorhamnetin on PAH remain unclear. This study aimed to investigate whether isorhamnetin has protective effects against PAH and explore possible mechanisms. An in vivo model of PAH induced by monocrotaline (MCT) was employed, and sildenafil and isorhamnetin were orally administered for 21 consecutive days. An in vitro model induced by TNF-alpha was employed, and cell proliferation of HPASMCs was detected. Results indicated that isorhamnetin significantly improved hemodynamic, histopathological, and echocardiographic changes in MCT-induced PAH in rats. In vitro, isorhamnetin suppressed TNF-alpha-induced HPASMCs proliferation. Furthermore, isorhamnetin improved protein expression of BMPR2 and suppressed protein expression of TNF-alpha and IL-6 in rat lungs. Isorhamnetin improved protein expression of BMPR2 and p-smad1/5 and mRNA expression of Id1 and Id3 in HPASMCs. Isorhamnetin ameliorated MCT-induced PAH in rats and inhibited TNF-alpha-induced HPASMCs proliferation by a mechanism likely involving the regulation of the BMP signaling pathway.
- Journal
- Phytother Res
- Publish Year
- 2020
- Experiment Subject
- rat
- Experiment Type
- Animal & Cell Experiment
- Phenotype Related
- Pulmonary Arterial Hypertension; Malignant Disease
- Paper Title Cn
- Paper Title En
- Protective effects of isorhamnetin on pulmonary arterial hypertension: in vivo and in vitro studies
- Bilingual Status
- semi_complete