ReferenceID 5502
Shikonin Attenuates Hepatic Steatosis by Enhancing Beta Oxidation and Energy Expenditure via AMPK Activation
Nutrients
Shikonin, a natural plant pigment, is known to have anti-obesity activity and to improve insulin sensitivity. This study aimed to examine the effect of shikonin on hepatic steatosis, focusing on the AMP-activated protein
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Record Fields
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- Reference Id
- 5502
- Evidence Id
- 22092
- Core Evidence Id
- 22092
- Source Reference Id
- 4248
- Herb2 Reference Id
- HBREF005045
- Subject Paper Key
- HBIN030442_32316687
- Pubmed Id
- 32316687
- Doi
- 10.3390/nu12041133
- Paper Title
- Shikonin Attenuates Hepatic Steatosis by Enhancing Beta Oxidation and Energy Expenditure via AMPK Activation
- Paper Abstract
- Shikonin, a natural plant pigment, is known to have anti-obesity activity and to improve insulin sensitivity. This study aimed to examine the effect of shikonin on hepatic steatosis, focusing on the AMP-activated protein kinase (AMPK) and energy expenditure in Hepa 1-6 cells and in high-fat fed mice. Shikonin increased AMPK phosphorylation in a dose- and time-dependent manner, and inhibition of AMPK with compound C inhibited this activation. In an oleic acid-induced steatosis model in hepatocytes, shikonin suppressed oleic acid-induced lipid accumulation, increased AMPK phosphorylation, suppressed the expression of lipogenic genes, and stimulated fatty acid oxidation-related genes. Shikonin administration for four weeks decreased body weight gain and the accumulation of lipid droplets in the liver of high-fat fed mice. Furthermore, shikonin promoted energy expenditure by activating fatty acid oxidation. In addition, shikonin increased the expression of PPARgamma coactivator-1alpha (PGC-1alpha), carnitine palmitoyltransferase-1 (CPT1) and other mitochondrial function-related genes. These results suggest that shikonin attenuated a high fat diet-induced nonalcoholic fatty liver disease by stimulating fatty acid oxidation and energy expenditure via AMPK activation.
- Journal
- Nutrients
- Publish Year
- 2020
- Experiment Subject
- mouse; hepa 1-6 cells
- Experiment Type
- Animal Experiment
- Phenotype Related
- Nonalcoholic Fatty Liver Disease
- Paper Title Cn
- Paper Title En
- Shikonin Attenuates Hepatic Steatosis by Enhancing Beta Oxidation and Energy Expenditure via AMPK Activation
- Bilingual Status
- semi_complete