ReferenceID 5491

Indirubin Derivatives as Dual Inhibitors Targeting Cyclin-Dependent Kinase and Histone Deacetylase for Treating Cancer

J Med Chem

To utilize the unique scaffold of a natural product indirubin, we herein adopted the strategy of combined pharmacophores to design and synthesize a series of novel indirubin derivatives as dual inhibitors against cyclin-

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Reference Id
5491
Evidence Id
22081
Core Evidence Id
22081
Source Reference Id
4230
Herb2 Reference Id
HBREF005027
Subject Paper Key
HBIN030108_34624191
Pubmed Id
34624191
Doi
10.1021/acs.jmedchem.1c01311
Paper Title
Indirubin Derivatives as Dual Inhibitors Targeting Cyclin-Dependent Kinase and Histone Deacetylase for Treating Cancer
Paper Abstract
To utilize the unique scaffold of a natural product indirubin, we herein adopted the strategy of combined pharmacophores to design and synthesize a series of novel indirubin derivatives as dual inhibitors against cyclin-dependent kinase (CDK) and histone deacetylase (HDAC). Among them, the lead compound 8b with remarkable CDK2/4/6 and HDAC6 inhibitory activity of IC50 = 60.9 +- 2.9, 276 +- 22.3, 27.2 +- 4.2, and 128.6 +- 0.4 nM, respectively, can efficiently induce apoptosis and S-phase arrest in several cancer cell lines. In particular, compound 8b can prevent the proliferation of a non-small-cell lung cancer cell line (A549) through the Mcl-1/XIAP/PARP axis, in agreement with the unique modes of action of the combined agents of HDAC inhibitors and CDK inhibitors. In an A549 xerograph model, compound 8b showed significant antitumor efficacy correlated with its dual inhibition. Our data demonstrated that compound 8b as a single agent could be a promising drug candidate for cancer therapy in combination with CDK and HDAC inhibitors.
Journal
J Med Chem
Publish Year
2021
Experiment Subject
Experiment Type
Cell Experiment
Phenotype Related
Non-small-cell Lung Cancer; Cancer
Paper Title Cn
Paper Title En
Indirubin Derivatives as Dual Inhibitors Targeting Cyclin-Dependent Kinase and Histone Deacetylase for Treating Cancer
Bilingual Status
semi_complete