ReferenceID 5479

Icariin attenuates endothelial-mesenchymal transition via H19/miR-148b-3p/ELF5 in ox-LDL-stimulated HUVECs

Mol Ther Nucleic Acids

Atherosclerosis is the main cause of cardio-cerebrovascular diseases. Endothelial-mesenchymal transition plays an important role in atherosclerosis. Icariin has a protective effect on atherosclerosis; however, the underl

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Reference Id
5479
Evidence Id
22069
Core Evidence Id
22069
Source Reference Id
4200
Herb2 Reference Id
HBREF004997
Subject Paper Key
HBIN029922_33510936
Pubmed Id
33510936
Doi
10.1016/j.omtn.2020.11.021
Paper Title
Icariin attenuates endothelial-mesenchymal transition via H19/miR-148b-3p/ELF5 in ox-LDL-stimulated HUVECs
Paper Abstract
Atherosclerosis is the main cause of cardio-cerebrovascular diseases. Endothelial-mesenchymal transition plays an important role in atherosclerosis. Icariin has a protective effect on atherosclerosis; however, the underlying mechanism remains unclear. In this study, we explored the molecular mechanism underlying the protective function of icariin in oxidized low-density lipoprotein-stimulated human umbilical vein endothelial cells. H19, a long non-coding RNA, was identified to be downregulated in the background of the oxidized low-density lipoprotein-induced endothelial-mesenchymal transition in human umbilical vein endothelial cells. Icariin upregulated H19 expression and inhibited the transformation of endothelial cells into interstitial cells. Overexpression of H19 affected endothelial-mesenchymal transition in oxidized low-density lipoprotein-stimulated human umbilical vein endothelial cells, whereas H19 knockdown reversed endothelial protective effects of icariin and reduced human umbilical vein endothelial cell migration. Knockdown of H19 significantly downregulated oxidized low-density lipoprotein-induced E74-like factor 5 and upregulated miR-148b-3p, which was reversed by icariin. Thus, icariin may play a protective role in atherosclerosis, and H19 may be a potential therapeutic target.
Journal
Mol Ther Nucleic Acids
Publish Year
2020
Experiment Subject
human; oxidized low-density lipoprotein-stimulated human umbilical vein endothelial cells
Experiment Type
Cell Experiment
Phenotype Related
Atherosclerosis; Cardio-cerebrovascular Diseases
Paper Title Cn
Paper Title En
Icariin attenuates endothelial-mesenchymal transition via H19/miR-148b-3p/ELF5 in ox-LDL-stimulated HUVECs
Bilingual Status
semi_complete