ReferenceID 5449
Apoptotic and antihepatofibrotic effect of honokiol via activation of GSK3β and suppression of Wnt/β-catenin pathway in hepatic stellate cells
Phytother Res
Though honokiol, derived from the Magnolia tree, was known to suppress renal fibrosis, pulmonary fibrosis, non-alcoholic steatoheptitis, inflammation and cancers, the underlying antifibrotic mechanisms of honokiol are no
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Record Fields
Scalar fields from the final reference record.
- Reference Id
- 5449
- Evidence Id
- 22039
- Core Evidence Id
- 22039
- Source Reference Id
- 4152
- Herb2 Reference Id
- HBREF004949
- Subject Paper Key
- HBIN029531_32776713
- Pubmed Id
- 32776713
- Doi
- 10.1002/ptr.6824
- Paper Title
- Apoptotic and antihepatofibrotic effect of honokiol via activation of GSK3β and suppression of Wnt/β-catenin pathway in hepatic stellate cells
- Paper Abstract
- Though honokiol, derived from the Magnolia tree, was known to suppress renal fibrosis, pulmonary fibrosis, non-alcoholic steatoheptitis, inflammation and cancers, the underlying antifibrotic mechanisms of honokiol are not fully understood in hepatic stellate cells until now. Thus, in the present study, inhibitory mechanism of honokiol on liver fibrosis was elucidated mainly in hepatic stellate cells (HSCs) by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, cell cycle analysis and western-blotting. Honokiol exerted cytotoxicity in LX-2, HSC-T6 and Hep-G2 cells. Honokiol increased sub G1 population and activated caspase 3 and cleaved poly (ADP-ribose) polymerase (PARP) in HSCs. Moreover, honokiol attenuated the expression of alpha smooth muscle actin (alpha-SMA), transforming growth factor beta 1 (TGF-beta1), phospho-Smad3, phospho-AKT, cyclin D1, c-Myc, Wnt3a, beta-catenin, and activated phosphorylation of glycogen synthase kinase 3 beta (GSK3beta) in HSCs. Conversely, GSK3beta inhibitor SB216763 reversed the effect of honokiol on PARP, alpha-SMA, phospho-GSK3beta, beta-catenin and sub G1 population in LX-2 cells. Overall, honokiol exerts apoptotic and antifibrotic effects via activation of GSK3beta and inhibition of Wnt3a/beta-catenin signalling pathway.
- Journal
- Phytother Res
- Publish Year
- 2021
- Experiment Subject
- Experiment Type
- Cell Experiment
- Phenotype Related
- Cancers; Inflammation; Liver Fibrosis; Renal Fibrosis; Non-alcoholic Steatoheptitis; Pulmonary Fibrosis
- Paper Title Cn
- Paper Title En
- Apoptotic and antihepatofibrotic effect of honokiol via activation of GSK3β and suppression of Wnt/β-catenin pathway in hepatic stellate cells
- Bilingual Status
- semi_complete