ReferenceID 5380
Glycyrrhizic acid suppresses early stage of adipogenesis through repression of MEK/ERK-mediated C/EBPβ and C/EBPδ expression in 3T3-L1 cells
Chem Biol Interact
Glycyrrhizic acid (GA), a major constituent of the root of licorice (Glycyrrhiza glabra), and has various biological activities, including anti-obesity property. However, the molecular mechanism of anti-adipogenic effect
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Record Fields
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- Reference Id
- 5380
- Evidence Id
- 21970
- Core Evidence Id
- 21970
- Source Reference Id
- 4009
- Herb2 Reference Id
- HBREF004806
- Subject Paper Key
- HBIN028190_34302803
- Pubmed Id
- 34302803
- Doi
- 10.1016/j.cbi.2021.109595
- Paper Title
- Glycyrrhizic acid suppresses early stage of adipogenesis through repression of MEK/ERK-mediated C/EBPβ and C/EBPδ expression in 3T3-L1 cells
- Paper Abstract
- Glycyrrhizic acid (GA), a major constituent of the root of licorice (Glycyrrhiza glabra), and has various biological activities, including anti-obesity property. However, the molecular mechanism of anti-adipogenic effect of GA is still unclear. In this study, we investigated the anti-adipogenic effects of GA in mouse adipocytic 3T3-L1 cells and elucidated its underlying molecular mechanism. GA decreased the intracellular triglyceride level. The expression levels of the adipogenic and lipogenic genes were lowered by treatment with GA in a concertation-dependent manner. In contrast, GA did not affect the lipolytic gene expression and the released glycerol level. GA suppressed the early stage of adipogenesis when it was added for 0-3 h after initiation of adipogenesis. Moreover, GA reduced the mRNA levels of CCAAT/enhancer binding protein (C/EBP) beta and C/EBPdelta, both of which activate the early stage of adipogenesis. Furthermore, GA decreased phosphorylation of extracellular signal-regulated kinase [ERK: p44/42 mitogen-activated protein kinase (MAPK)] in the early stage of adipogenesis. In addition, a MAPK kinase (MEK) inhibitor, PD98059 reduced the C/EBPbeta and C/EBPdelta gene expression. These results indicate that GA suppressed the early stage of adipogenesis through repressing the MEK/ERK-mediated C/EBPbeta and C/EBPdelta expression in 3T3-L1 cells. Thus, GA has an anti-adipogenic ability and a possible agent for treatment of obesity.
- Journal
- Chem Biol Interact
- Publish Year
- 2021
- Experiment Subject
- mouse; 3t3-l1 cells; licorice; mouse adipocytic 3t3-l1 cells
- Experiment Type
- Cell Experiment
- Phenotype Related
- Obesity
- Paper Title Cn
- Paper Title En
- Glycyrrhizic acid suppresses early stage of adipogenesis through repression of MEK/ERK-mediated C/EBPβ and C/EBPδ expression in 3T3-L1 cells
- Bilingual Status
- semi_complete