ReferenceID 5313
Geniposide ameliorated dexamethasone-induced endoplasmic reticulum stress and mitochondrial apoptosis in osteoblasts
J Ethnopharmacol
Ethnopharmacological relevance: Eucommia ulmoides Oliver has been traditionally used for treatment of various diseases, including osteoporosis, knee pain, and paralysis. The extract of Eucommia ulmoides has been reported
Relationship Network
Interactive first-hop connections across herbs, ingredients, formulas, targets, diseases, symptoms, syndromes, evidence, and monographs.
Click a node to open it in a new tab
Ingredient: 1Reference: 1Links: 1
Arranging relationship network...
Record Fields
Scalar fields from the final reference record.
- Reference Id
- 5313
- Evidence Id
- 21903
- Core Evidence Id
- 21903
- Source Reference Id
- 3877
- Herb2 Reference Id
- HBREF004674
- Subject Paper Key
- HBIN027450_35240241
- Pubmed Id
- 35240241
- Doi
- 10.1016/j.jep.2022.115154
- Paper Title
- Geniposide ameliorated dexamethasone-induced endoplasmic reticulum stress and mitochondrial apoptosis in osteoblasts
- Paper Abstract
- Ethnopharmacological relevance: Eucommia ulmoides Oliver has been traditionally used for treatment of various diseases, including osteoporosis, knee pain, and paralysis. The extract of Eucommia ulmoides has been reported to stimulate the bone formation and suppress the bone resorption, leading to protection against osteoporosis (OP). Geniposide (GEN) has been considered as one of the effective compounds responsible for the therapeutic efficacy of Eucommia ulmoides against OP. Aim of the study: To explore whether GEN protected against dexamethasone (DEX)-induced osteoporosis (OP) by activating NRF2 expression and inhibiting endoplasmic reticulum (ER) stress. Materials and methods: The DEX-induced rat OP models were duplicated. The pathological changes were examined by histological/immunohistochemical evaluation and micro-computed tomography (micro-CT) assessment. Apoptosis was detected by a flow cytometer. Mitochondrial Ca 2+ concentrations and mitochondrial membrane potential were detected. Western blot assays were used to detect the protein expression. Results: GEN effectively reversed DEX-induced pathological changes of trabecular bone in rats. In addition, the DEX-increased expression of ATF4/CHOP was also ameliorated. In MC3T3-E1 cells, DEX promoted endoplasmic reticulum (ER) stress and mitochondrial apoptosis. Inhibition of ER stress abolished the induction of apoptosis by DEX. Similarly, GEN significantly ameliorated DEX-induced mitochondrial apoptosis. The possible underlying mechanism might be associated with the pharmacological effects of GEN on activating the expression of NRF2 and alleviating ER stress in DEX-treated MC3T3-E1 cells. Conclusion: GEN ameliorated DEX-induced ER stress and mitochondrial apoptosis in osteoblasts.
- Journal
- J Ethnopharmacol
- Publish Year
- 2022
- Experiment Subject
- rat; dex-treated mc3t3-e1 cells; mc3t3-e1 cells
- Experiment Type
- Animal Experiment
- Phenotype Related
- Paralysis; Eucommia Ulmoides; Osteoporosis; Knee Pain
- Paper Title Cn
- Paper Title En
- Geniposide ameliorated dexamethasone-induced endoplasmic reticulum stress and mitochondrial apoptosis in osteoblasts
- Bilingual Status
- semi_complete