ReferenceID 5261
Formononetin inhibits IL-1β-induced inflammation in human chondrocytes and slows the progression of osteoarthritis in rat model via the regulation of PTEN/AKT/NF-κB pathway
Int Immunopharmacol
Background/aim: Osteoarthritis (OA) is a common degenerative disease characterized by cartilage degradation and inflammation. This study aimed to investigate the anti-inflammatory properties of formononetin, an isoflavon
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Record Fields
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- Reference Id
- 5261
- Evidence Id
- 21851
- Core Evidence Id
- 21851
- Source Reference Id
- 3785
- Herb2 Reference Id
- HBREF004582
- Subject Paper Key
- HBIN026662_36306560
- Pubmed Id
- 36306560
- Doi
- 10.1016/j.intimp.2022.109309
- Paper Title
- Formononetin inhibits IL-1β-induced inflammation in human chondrocytes and slows the progression of osteoarthritis in rat model via the regulation of PTEN/AKT/NF-κB pathway
- Paper Abstract
- Background/aim: Osteoarthritis (OA) is a common degenerative disease characterized by cartilage degradation and inflammation. This study aimed to investigate the anti-inflammatory properties of formononetin, an isoflavone extracted from astragalus membranaceus, on OA. Methods: Human OA chondrocytes were pretreated in vitro with formononetin and subsequently stimulated with IL-1β. The production of inflammatory mediators, cytokines and the synthesis of catabolic factors were evaluated by ELISA and Western blot analysis. In addition, a rat model of OA was established and treated with formononetin. Results: Formononetin attenuated the overproduction of inflammatory mediators and cytokines, suppressed the expression of cyclooxygenase-2 and inducible nitric oxide synthase, and inhibited the synthesis of catabolic factors such as MMPs and thrombospondin motifs 5. Furthermore, formononetin exerted protective effects in a rat model of OA. Mechanistically, we found that formononetin inhibited IL-1β induced activation of nuclear factor kappa B and AKT by activating the phosphatase and tensin homolog. Conclusions: Formononetin could be used as a potential agent for OA treatment.
- Journal
- Int Immunopharmacol
- Publish Year
- 2022
- Experiment Subject
- rat; human
- Experiment Type
- Animal & Cell Experiment
- Phenotype Related
- Osteoarthritis; Inflammation; Degenerative Disease
- Paper Title Cn
- Paper Title En
- Formononetin inhibits IL-1β-induced inflammation in human chondrocytes and slows the progression of osteoarthritis in rat model via the regulation of PTEN/AKT/NF-κB pathway
- Bilingual Status
- semi_complete