ReferenceID 5248
Fisetin Attenuated Oxidative Stress-Induced Cellular Damage in ARPE-19 Human Retinal Pigment Epithelial Cells Through Nrf2-Mediated Activation of Heme Oxygenase-1
Front Pharmacol
Fisetin is a kind of bioactive flavonol, widely present in various fruits such as strawberries and apples, and is known to act as a potent free radical scavenger. However, the mechanism of action related to the antioxida
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Record Fields
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- Reference Id
- 5248
- Evidence Id
- 21838
- Core Evidence Id
- 21838
- Source Reference Id
- 3764
- Herb2 Reference Id
- HBREF004561
- Subject Paper Key
- HBIN026506_35784747
- Pubmed Id
- 35784747
- Doi
- 10.3389/fphar.2022.927898
- Paper Title
- Fisetin Attenuated Oxidative Stress-Induced Cellular Damage in ARPE-19 Human Retinal Pigment Epithelial Cells Through Nrf2-Mediated Activation of Heme Oxygenase-1
- Paper Abstract
- Fisetin is a kind of bioactive flavonol, widely present in various fruits such as strawberries and apples, and is known to act as a potent free radical scavenger. However, the mechanism of action related to the antioxidant activity of this compound in human retinal pigment epithelial (RPE) cells is not precisely known. In this study, we aimed to investigate whether fisetin could attenuate oxidative stress-induced cytotoxicity on human RPE ARPE-19 cells. To mimic oxidative stress, ARPE-19 cells were treated with hydrogen peroxide (H 2 O 2 ), and fisetin significantly inhibited H 2 O 2 -induced loss of cell viability and increase of intracellular reactive oxygen species (ROS) production. Fisetin also markedly attenuated DNA damage and apoptosis in H 2 O 2 -treated ARPE-19 cells. Moreover, mitochondrial dysfunction in H 2 O 2 -treated cells was alleviated in the presence of fisetin as indicated by preservation of mitochondrial membrane potential, increase of Bcl-2/Bax expression ratio, and suppression of cytochrome c release into the cytoplasm. In addition, fisetin enhanced phosphorylation and nuclear translocation of nuclear factor erythroid 2 related factor 2 (Nrf2), which was associated with increased expression and activity of heme oxygenase-1 (HO-1). However, the HO-1 inhibitor, zinc protoporphyrin, significantly reversed the protective effect of fisetin against H 2 O 2 -mediated ARPE-19 cell injury. Therefore, our results suggest that Nrf2-mediated activation of antioxidant enzyme HO-1 may play an important role in the ROS scavenging activity of fisetin in RPE cells, contributing to the amelioration of oxidative stress-induced ocular disorders.
- Journal
- Front Pharmacol
- Publish Year
- 2022
- Experiment Subject
- human; arpe-19 cells; h 2 o 2 -treated arpe-19 cells; h 2 o 2 -treated cells; human rpe arpe-19 cells
- Experiment Type
- Cell Experiment
- Phenotype Related
- Ocular Disorders
- Paper Title Cn
- Paper Title En
- Fisetin Attenuated Oxidative Stress-Induced Cellular Damage in ARPE-19 Human Retinal Pigment Epithelial Cells Through Nrf2-Mediated Activation of Heme Oxygenase-1
- Bilingual Status
- semi_complete