ReferenceID 5214
Falcarindiol Purified From Carrots Leads to Elevated Levels of Lipid Droplets and Upregulation of Peroxisome Proliferator-Activated Receptor-γ Gene Expression in Cellular Models
Front Pharmacol
Falcarindiol (FaDOH) is a cytotoxic and anti-inflammatory polyacetylenic oxylipin found in food plants of the carrot family (Apiaceae). FaDOH has been shown to activate PPARgamma and to increase the expression of the cho
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Record Fields
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- Reference Id
- 5214
- Evidence Id
- 21804
- Core Evidence Id
- 21804
- Source Reference Id
- 3705
- Herb2 Reference Id
- HBREF004502
- Subject Paper Key
- HBIN026343_32982759
- Pubmed Id
- 32982759
- Doi
- 10.3389/fphar.2020.565524
- Paper Title
- Falcarindiol Purified From Carrots Leads to Elevated Levels of Lipid Droplets and Upregulation of Peroxisome Proliferator-Activated Receptor-γ Gene Expression in Cellular Models
- Paper Abstract
- Falcarindiol (FaDOH) is a cytotoxic and anti-inflammatory polyacetylenic oxylipin found in food plants of the carrot family (Apiaceae). FaDOH has been shown to activate PPARgamma and to increase the expression of the cholesterol transporter ABCA1 in cells, both of which play an important role in lipid metabolism. Thus, a common mechanism of action of the anticancer and antidiabetic properties of FaDOH may be due to a possible effect on lipid metabolism. In this study, the effect of sub-toxic concentration (5 muM) of FaDOH inside human mesenchymal stem cells (hMSCs) was studied using white light microscopy and Raman imaging. Our results show that FaDOH increases lipid content in the hMSCs cells as well as the number of lipid droplets (LDs) and that this can be explained by increased expression of PPARgamma2 as shown in human colon adenocarcinoma cells. Activation of PPARgamma can lead to increased expression of ABCA1. We demonstrate that ABCA1 is upregulated in colorectal neoplastic rat tissue, which indicates a possible role of this transporter in the redistribution of lipids and increased formation of LDs in cancer cells that may lead to endoplasmic reticulum stress and cancer cell death.
- Journal
- Front Pharmacol
- Publish Year
- 2020
- Experiment Subject
- rat; human; carrot; hmscs cells
- Experiment Type
- Cell Experiment
- Phenotype Related
- Colon Adenocarcinoma; Cancer
- Paper Title Cn
- Paper Title En
- Falcarindiol Purified From Carrots Leads to Elevated Levels of Lipid Droplets and Upregulation of Peroxisome Proliferator-Activated Receptor-γ Gene Expression in Cellular Models
- Bilingual Status
- semi_complete