ReferenceID 5212

The Protective Effect of Evodiamine in Osteoarthritis: An In Vitro and In Vivo Study in Mice Model

Front Pharmacol

Osteoarthritis (OA) is a chronic disease with high economic burden characterized by cartilage degradation and joint inflammation. Evodiamine (EV), which can be extracted from Evodia rutaecarpa (Rutaceae), is a traditiona

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Reference Id
5212
Evidence Id
21802
Core Evidence Id
21802
Source Reference Id
3703
Herb2 Reference Id
HBREF004500
Subject Paper Key
HBIN026264_35795554
Pubmed Id
35795554
Doi
10.3389/fphar.2022.899108
Paper Title
The Protective Effect of Evodiamine in Osteoarthritis: An In Vitro and In Vivo Study in Mice Model
Paper Abstract
Osteoarthritis (OA) is a chronic disease with high economic burden characterized by cartilage degradation and joint inflammation. Evodiamine (EV), which can be extracted from Evodia rutaecarpa (Rutaceae), is a traditional Chinese medicine to treat inflammation, cardiovascular disorders, infection, and obesity. Studies have shown that EV can suppress the activation of immune cells and restrain the secretion of pro-inflammatory cytokines. However, it is still not well known about its role in the treatment of OA. In this study, we utilized interleukin-1β (IL-1β)-stimulated mouse chondrocytes in vitro and the destabilization of the medial meniscus (DMM) model in vivo to demonstrate the anti-inflammatory properties of EV in OA. The results suggested that EV decreased the generation of NO, IL-6, TNF-α, and PGE2. Meanwhile, the increased expression of iNOS, COX-2, and MMP-13 and the degradation of aggrecan and Col-II were significantly alleviated by EV in IL-1β-activated mouse chondrocytes. Moreover, EV can inhibit the considerable IL-1β-stimulated phosphorylation of the NF-κB signaling pathway and nuclear translocation of p65, compared with the control group. Furthermore, EV alleviated cartilage degeneration and reversed the increased Osteoarthritis Research Society International (OARSI) scores in the OA model in vivo . Our study demonstrates that EV can suppress inflammation in vitro and cartilage degeneration in vivo in OA, which implies that EV may be a potential candidate for the treatment of OA.
Journal
Front Pharmacol
Publish Year
2022
Experiment Subject
mouse; il-1β-activated mouse chondrocytes
Experiment Type
Animal & Cell Experiment
Phenotype Related
Joint Inflammation; Obesity; Inflammation; Chronic Disease; Cardiovascular Disorders; Cartilage Degeneration; Osteoarthritis; Infection
Paper Title Cn
Paper Title En
The Protective Effect of Evodiamine in Osteoarthritis: An In Vitro and In Vivo Study in Mice Model
Bilingual Status
semi_complete