ReferenceID 5199
Eupatilin prevents behavioral deficits and dopaminergic neuron degeneration in a Parkinson's disease mouse model
Life Sci
AIMS: Neuroinflammation and apoptosis play a crucial role in Parkinson's disease (PD) pathogenesis. Eupatilin is a lipophilic flavonoid isolated from Artemisia species and exerts anti-apoptotic and anti-inflammatory acti
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- Reference Id
- 5199
- Evidence Id
- 21789
- Core Evidence Id
- 21789
- Source Reference Id
- 3686
- Herb2 Reference Id
- HBREF004483
- Subject Paper Key
- HBIN026145_32376269
- Pubmed Id
- 32376269
- Doi
- 10.1016/j.lfs.2020.117745
- Paper Title
- Eupatilin prevents behavioral deficits and dopaminergic neuron degeneration in a Parkinson's disease mouse model
- Paper Abstract
- AIMS: Neuroinflammation and apoptosis play a crucial role in Parkinson's disease (PD) pathogenesis. Eupatilin is a lipophilic flavonoid isolated from Artemisia species and exerts anti-apoptotic and anti-inflammatory activities. In this study, we investigated the effects of Eupatilin on a mouse model of PD induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). MAIN METHODS: The rotarod test and traction test were constructed to examine the motor function. Immunofluorescent staining was performed to detect the expression of TH, Iba-1 and GFAP. Apoptosis was examined by the TUNEL assay. Real-time PCR was used to determine the mRNA expression and Western blot and ELISA were used to determine the protein expression. KEY FINDINGS: Eupatilin improved behavioral impairment caused by MPTP. A loss of TH positive neurons was observed in the substantia nigra pars compacta of MPTP-lesioned brain, while it was rescued by Eupatilin. Moreover, MPTP administration increased the cell number of microglia and astrocytes and the expression of inflammatory factors TNF-alpha, IL-1beta, and IL-6. Whereas Eupatilin suppressed the activation of neuroinflammation. Eupatilin also decreased cell apoptosis enhanced by MPTP/MPP+ exposure in vivo and in vitro. We further revealed that Eupatilin abolished MPTP-induced downregulation of IkappaBalpha expression and accumulation of p65 in the nuclear compartment. Besides, MPTP administration led to dephosphorylation of Akt and GSK-3beta, but it was restored by Eupatilin. SIGNIFICANCE: We demonstrate that Eupatilin alleviates behavioral impairment and dopaminergic neuron loss induced by MPTP through inhibition of neuroinflammation and apoptosis. Our research provides more evidence for Eupatilin as a potential preventative drug for PD.
- Journal
- Life Sci
- Publish Year
- 2020
- Experiment Subject
- mouse
- Experiment Type
- Animal Experiment
- Phenotype Related
- Parkinson's Disease; Neuroinflammation
- Paper Title Cn
- Paper Title En
- Eupatilin prevents behavioral deficits and dopaminergic neuron degeneration in a Parkinson's disease mouse model
- Bilingual Status
- semi_complete