ReferenceID 5191
Effects of Erythrodiol on the Antioxidant Response and Proteome of HepG2 Cells
Antioxidants (Basel)
Erythrodiol (EO) is a pentacyclic triterpenic alcohol found in olive tree leaves and olive oil, and it has important effects on the health properties and quality of olive oil. In this study, we characterized the cytotoxi
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Record Fields
Scalar fields from the final reference record.
- Reference Id
- 5191
- Evidence Id
- 21781
- Core Evidence Id
- 21781
- Source Reference Id
- 3666
- Herb2 Reference Id
- HBREF004463
- Subject Paper Key
- HBIN025716_35052578
- Pubmed Id
- 35052578
- Doi
- 10.3390/antiox11010073
- Paper Title
- Effects of Erythrodiol on the Antioxidant Response and Proteome of HepG2 Cells
- Paper Abstract
- Erythrodiol (EO) is a pentacyclic triterpenic alcohol found in olive tree leaves and olive oil, and it has important effects on the health properties and quality of olive oil. In this study, we characterized the cytotoxic effects of EO on human hepatocarcinoma (HepG2) cells by studying changes in cell viability, reactive oxygen species (ROS) production, antioxidant defense systems, and the proteome. The results reveal that EO markedly decreased HepG2 cell viability without changing ROS levels. The concentrations of glutathione and NADPH were significantly reduced, with selective changes in the activity of several antioxidant enzymes: glutathione peroxidase, glutathione reductase, glucose 6-phosphate dehydrogenase, and 6-phosphogluconate dehydrogenase. Proteomic data reveal that EO led to the complete elimination or decreased abundance of 41 and 3 proteins, respectively, and the abundance of 29 proteins increased. The results of functional enrichment analysis show that important metabolic processes and the nuclear transport of mature mRNA were impaired, whereas AMP biosynthesis and cell cycle G2/M phase transition were induced. The transcription factors and miRNAs involved in this response were also identified. These potent antiproliferative effects make EO a good candidate for the further analysis of its hepatic antitumor effects in in vivo studies.
- Journal
- Antioxidants (Basel)
- Publish Year
- 2021
- Experiment Subject
- human; hepg2 cell; human hepatocarcinoma (hepg2) cells
- Experiment Type
- Cell Experiment
- Phenotype Related
- Hepatocarcinoma
- Paper Title Cn
- Paper Title En
- Effects of Erythrodiol on the Antioxidant Response and Proteome of HepG2 Cells
- Bilingual Status
- semi_complete