ReferenceID 5119
Dieckol Inhibits Autophagic Flux and Induces Apoptotic Cell Death in A375 Human Melanoma Cells via Lysosomal Dysfunction and Mitochondrial Membrane Impairment
Int J Mol Sci
Dieckol is a natural brown algal-derived polyphenol and its cytotoxic potential against various types of cancer cells has been studied. However, the effects of dieckol on autophagy in cancer cells remain unknown. Here, w
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Record Fields
Scalar fields from the final reference record.
- Reference Id
- 5119
- Evidence Id
- 21709
- Core Evidence Id
- 21709
- Source Reference Id
- 3523
- Herb2 Reference Id
- HBREF004320
- Subject Paper Key
- HBIN023738_36430634
- Pubmed Id
- 36430634
- Doi
- 10.3390/ijms232214149
- Paper Title
- Dieckol Inhibits Autophagic Flux and Induces Apoptotic Cell Death in A375 Human Melanoma Cells via Lysosomal Dysfunction and Mitochondrial Membrane Impairment
- Paper Abstract
- Dieckol is a natural brown algal-derived polyphenol and its cytotoxic potential against various types of cancer cells has been studied. However, the effects of dieckol on autophagy in cancer cells remain unknown. Here, we show that dieckol inhibits the growth of A375 human melanoma cells by inducing apoptotic cell death, which is associated with lysosomal dysfunction and the inhibition of autophagic flux. Dieckol induces autophagosome accumulation by inhibiting autophagosome-lysosome fusion. Moreover, dieckol not only triggers lysosomal membrane permeabilization, followed by an increase in lysosomal pH and the inactivation of cathepsin B and D, but also causes the loss of mitochondrial membrane potential. Importantly, a cathepsin D inhibitor partially relieved dieckol-induced mitochondrial membrane impairment and caspase-mediated apoptosis. Collectively, our findings indicate that dieckol is a novel autophagy inhibitor that induces apoptosis-mediated cell death via lysosomal dysfunction and mitochondrial membrane impairment in A375 human melanoma cells. This suggests the novel potential value of dieckol as a chemotherapeutic drug candidate for melanoma treatment.
- Journal
- Int J Mol Sci
- Publish Year
- 2022
- Experiment Subject
- human; a375 human melanoma cells
- Experiment Type
- Cell Experiment
- Phenotype Related
- Melanoma; Cancer; Mitochondrial Membrane Impairment; Lysosomal Dysfunction
- Paper Title Cn
- Paper Title En
- Dieckol Inhibits Autophagic Flux and Induces Apoptotic Cell Death in A375 Human Melanoma Cells via Lysosomal Dysfunction and Mitochondrial Membrane Impairment
- Bilingual Status
- semi_complete