ReferenceID 5109
Effects of dibutyl phthalate on lipid metabolism in liver and hepatocytes based on PPARα/SREBP-1c/FAS/GPAT/AMPK signal pathway
Food Chem Toxicol
Phateacid esters (PAEs), such as dibutyl phthalate (DBP), have been widely used and human exposure results into serious toxic effects; such as the development of fatty liver disease. In the present study, SD rat models f
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Record Fields
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- Reference Id
- 5109
- Evidence Id
- 21699
- Core Evidence Id
- 21699
- Source Reference Id
- 3506
- Herb2 Reference Id
- HBREF004303
- Subject Paper Key
- HBIN023631_33508418
- Pubmed Id
- 33508418
- Doi
- 10.1016/j.fct.2021.112029
- Paper Title
- Effects of dibutyl phthalate on lipid metabolism in liver and hepatocytes based on PPARα/SREBP-1c/FAS/GPAT/AMPK signal pathway
- Paper Abstract
- Phateacid esters (PAEs), such as dibutyl phthalate (DBP), have been widely used and human exposure results into serious toxic effects; such as the development of fatty liver disease. In the present study, SD rat models for in vivo study (normal and fatty liver model group) and hepatocytes for in vitro study (normal and abnormal lipid metabolism model group) were established to determine the effects of DBP on liver function and discover the possible mechanisms. Meanwhile, the peroxisome proliferator activated receptor (PPARalpha) blocker, GW6471, with the Adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK) activator, AICAR, were applied in vitro study to clarify the role of PPARalpha/SREBP-1c/FAS/GPAT/AMPK signal pathway in the process. Results suggested that DBP could activate PPARalpha signaling pathway and affected the protein expression of SREBP, FAS and GPAT to cause hyperlipidemia and abnormal liver function. DBP also could inhibit the phosphorylation and activation of AMPK to inhibit the decomposition and metabolism of lipids. Interestingly, the effects of DBP could be alleviated by GW6471 and AICAR. Our experimental results provide reliable evidence that DBP exposure could further induce liver lipid metabolism disorder and other hepatic toxicity through PPARalpha/SREBP-1c/FAS/GPAT/AMPK signal pathway.
- Journal
- Food Chem Toxicol
- Publish Year
- 2021
- Experiment Subject
- rat; human
- Experiment Type
- Cell Experiment
- Phenotype Related
- Fatty Liver Disease; Liver Lipid Metabolism Disorder; Hyperlipidemia
- Paper Title Cn
- Paper Title En
- Effects of dibutyl phthalate on lipid metabolism in liver and hepatocytes based on PPARα/SREBP-1c/FAS/GPAT/AMPK signal pathway
- Bilingual Status
- semi_complete