ReferenceID 5108
Insight into drug sensitizing effect of diallyl disulfide and diallyl trisulfide from Allium sativum L. on paclitaxel-resistant triple-negative breast cancer cells
J Ethnopharmacol
Ethnopharmacological relevance: Ayurvedic practitioners and herbal healers in India and China have extensively used garlic (Allium sativum L.) to treat cancers. Diallyl disulfide (DADS) and diallyl trisulfide (DATS) are
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Record Fields
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- Reference Id
- 5108
- Evidence Id
- 21698
- Core Evidence Id
- 21698
- Source Reference Id
- 3505
- Herb2 Reference Id
- HBREF004302
- Subject Paper Key
- HBIN023556_35690339
- Pubmed Id
- 35690339
- Doi
- 10.1016/j.jep.2022.115452
- Paper Title
- Insight into drug sensitizing effect of diallyl disulfide and diallyl trisulfide from Allium sativum L. on paclitaxel-resistant triple-negative breast cancer cells
- Paper Abstract
- Ethnopharmacological relevance: Ayurvedic practitioners and herbal healers in India and China have extensively used garlic (Allium sativum L.) to treat cancers. Diallyl disulfide (DADS) and diallyl trisulfide (DATS) are major volatile organosulfur phytochemical constituents found in garlic. Aim of the study: To find new insight into the drug sensitizing effect of DADS and DATS on paclitaxel (PTX)-resistant triple-negative breast cancer cells (TNBC/PR). Materials and methods: This study estimates the non-toxic concentration of DADS and DATS against normal healthy breast epithelial cell line (MCF-12A) by using a trypan blue viability assay. Also, it evaluates the effect of DADS and DATS on the sensitization of established stable TNBC/PR cell clones (MDA-MB 231 PR and MDA-MB 468 PR) by MTT, BrdU incorporation, intracellular ROS, cell cycle, and apoptosis assays. Results: The results show that DADS and DATS are non-cytotoxicity against MCF-12A cells. Nevertheless, DADS and DATS have shown significantly high cytotoxicity against MDA-MB 231 PR and MDA-MB 468 PR cells. They also inhibited PTX-resistant cell proliferation by blocking the cell cycle. Further, they induced apoptosis by activation of caspase 3 and 9. N-acetyl cysteine pre-treatment inhibited DADS and DATS-induced intracellular ROS release. In silico study shows that DADS and DATS interact with a large extracellular loop (LEL) of CD151 with a binding energy of -4.0 kcal/mol and transmembrane domain (TM) with a binding affinity of 11.7 and 13.6 kcal/mol, respectively. They also inhibited the surface expression of CD151 in TNBC/PR cells. Conclusion: This study implies that DADS and DATS could be considered for sensitizing drug-resistant breast cancers.
- Journal
- J Ethnopharmacol
- Publish Year
- 2022
- Experiment Subject
- garlic; mcf-12a cells; mda-mb 468 pr cells; normal healthy breast epithelial cell line; paclitaxel (ptx)-resistant triple-negative breast cancer cells; tnbc/pr cell clones; tnbc/pr cells
- Experiment Type
- Cell Experiment
- Phenotype Related
- Drug-resistant Breast Cancers; Triple-negative Breast Cancer; Cancers
- Paper Title Cn
- Paper Title En
- Insight into drug sensitizing effect of diallyl disulfide and diallyl trisulfide from Allium sativum L. on paclitaxel-resistant triple-negative breast cancer cells
- Bilingual Status
- semi_complete