ReferenceID 5064
Gamma-oryzanol alleviates intervertebral disc degeneration development by intercepting the IL-1β/NLRP3 inflammasome positive cycle
Phytomedicine
Background: Intervertebral disc degeneration (IVDD) is a highly prevalent musculoskeletal disorder characterized by a local inflammatory response associated with the IL-1β/NLRP3 inflammasome positive feedback loop. Rice
Relationship Network
Interactive first-hop connections across herbs, ingredients, formulas, targets, diseases, symptoms, syndromes, evidence, and monographs.
Click a node to open it in a new tab
Ingredient: 1Reference: 1Links: 1
Arranging relationship network...
Record Fields
Scalar fields from the final reference record.
- Reference Id
- 5064
- Evidence Id
- 21654
- Core Evidence Id
- 21654
- Source Reference Id
- 3404
- Herb2 Reference Id
- HBREF004201
- Subject Paper Key
- HBIN022150_35660354
- Pubmed Id
- 35660354
- Doi
- 10.1016/j.phymed.2022.154176
- Paper Title
- Gamma-oryzanol alleviates intervertebral disc degeneration development by intercepting the IL-1β/NLRP3 inflammasome positive cycle
- Paper Abstract
- Background: Intervertebral disc degeneration (IVDD) is a highly prevalent musculoskeletal disorder characterized by a local inflammatory response associated with the IL-1β/NLRP3 inflammasome positive feedback loop. Rice bran-derived gamma-oryzanol (Ory) as a sterol ferulate has attracted much attention due to its powerful anti-inflammatory, hypoglycemic and hypolipidemic health effects. As a clinical pharmaceutical for autonomic disorders, Ory's role in musculoskeletal degenerative disease remains unknown. Purpose: This study aims to validate the role of Ory in IVDD and explore the potential mechanism. Study design: Establishing the in vitro and in vivo IVDD models to detect the protective effect and molecular mechanism of Ory. Method: The anti-ECM degradation, antioxidant and anti-NLRP3 inflammasome activation effects of Ory on IL-1β-stimulated nucleus pulposus (NP) cells were assessed by immunoblotting and immunofluorescence, etc. MRI, S-O staining and immunohistochemistry were performed to estimate the effects of Ory administration on acupuncture-mediated IVDD in rats at imaging and histological levels. Results: Ory treatment inhibited IL-1β-mediated ECM degradation, oxidative stress and NLRP3 inflammasome activation in NP cells. By interfering with NF-κB signaling and ROS overproduction, Ory interrupted IL-1β/NLRP3-inflammasome positive cycle. In vivo experiments showed that Ory delayed acupuncture-mediated IVDD development. Conclusion: Our results support the potential application of Ory as a therapeutic compound for IVDD.
- Journal
- Phytomedicine
- Publish Year
- 2022
- Experiment Subject
- rat; il-1β-stimulated nucleus pulposus (np) cells
- Experiment Type
- Animal Experiment
- Phenotype Related
- Musculoskeletal Degenerative Disease; Musculoskeletal Disorder; Autonomic Disorders; Intervertebral Disc Degeneration
- Paper Title Cn
- Paper Title En
- Gamma-oryzanol alleviates intervertebral disc degeneration development by intercepting the IL-1β/NLRP3 inflammasome positive cycle
- Bilingual Status
- semi_complete