ReferenceID 5001
Curculigoside inhibits ferroptosis in ulcerative colitis through the induction of GPX4
Life Sci
Curculigoside (CUR) is natural ingredient from Curculigo orchioides Gaertn with multiple biological activities. However, whether CUR protects from ulcerative colitis (UC) and underlying mechanisms are unclear. Herein, mi
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Record Fields
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- Reference Id
- 5001
- Evidence Id
- 21591
- Core Evidence Id
- 21591
- Source Reference Id
- 3255
- Herb2 Reference Id
- HBREF004052
- Subject Paper Key
- HBIN021964_32861798
- Pubmed Id
- 32861798
- Doi
- 10.1016/j.lfs.2020.118356
- Paper Title
- Curculigoside inhibits ferroptosis in ulcerative colitis through the induction of GPX4
- Paper Abstract
- Curculigoside (CUR) is natural ingredient from Curculigo orchioides Gaertn with multiple biological activities. However, whether CUR protects from ulcerative colitis (UC) and underlying mechanisms are unclear. Herein, mice challenged with dextran sulfate sodium (DSS) were established and administrated with CUR for 7 days. Then histological pathologies and ferroptosis regulators were determined in vivo. The ferroptotic IEC-6 cells were prepared to investigate the underlying mechanism of CUR. Results showed that CUR inhibited the disease activity index, histological damage and cell death in mice with colitis. We also found that ferroptosis was induced in mice with colitis, as evidenced by iron overload, GSH depletion, ROS and MDA production, accompanied by decreased expression of SOD and GPX4. CUR treatment significantly reversed these alterations of ferroptotic features in DSS-induced mice. Furthermore, similar effects of CUR on ferroptosis were observed in IEC-6 cells under the combined treatment of H2O2 and iron chloride hexahydrate. Interestingly, we found that CUR could increase the selenium sensitivity and promote GPX4 transcription level in IEC-6 cells. Knockdown of GPX4 significantly blocked the protective effects of CUR on cell death, GSH and MDA contents as well as LDH activity in ferroptotic IEC-6 cells. Taken together, these findings suggest that CUR protects against ferroptosis in UC by the induction of GPX4, which presents a potential agent for UC treatment.
- Journal
- Life Sci
- Publish Year
- 2020
- Experiment Subject
- mouse; ferroptotic iec-6 cells; iec-6 cells
- Experiment Type
- Animal & Cell Experiment
- Phenotype Related
- Ferroptosis; Ulcerative Colitis; Colitis
- Paper Title Cn
- Paper Title En
- Curculigoside inhibits ferroptosis in ulcerative colitis through the induction of GPX4
- Bilingual Status
- semi_complete