ReferenceID 4989

Cryptotanshinone enhances neurite outgrowth and memory via extracellular signal-regulated kinase 1/2 signaling

Food Chem Toxicol

Neurite outgrowth is important process in synaptic formation and neuronal development. Many previous studies reported that natural compounds as well as neurotrophins induce neurite outgrowth through various signaling pat

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Reference Id
4989
Evidence Id
21579
Core Evidence Id
21579
Source Reference Id
3224
Herb2 Reference Id
HBREF004021
Subject Paper Key
HBIN021795_31790773
Pubmed Id
31790773
Doi
10.1016/j.fct.2019.111011
Paper Title
Cryptotanshinone enhances neurite outgrowth and memory via extracellular signal-regulated kinase 1/2 signaling
Paper Abstract
Neurite outgrowth is important process in synaptic formation and neuronal development. Many previous studies reported that natural compounds as well as neurotrophins induce neurite outgrowth through various signaling pathways. In this study, we tested the effect of cryptotanshinone (CPT), a constituent of Salvia miltiorrhiza Bunge, on neurite outgrowth using neuro2a cell line, a mouse neuroblastoma cell line. And then, we examined the effect of CPT on learning and memory. We first found that CPT facilitated neurite outgrowth in a concentration-dependent manner. Although CPT induced MTT reduction, CPT did not induce LDH release. Moreover, CPT suppressed cell proliferation. CPT increased ERK1/2 phosphorylation and ERK1/2 inhibitor blocked CPT-facilitated neurite outgrowth. CPT also enhanced learning and memory without affecting basal sensory conditions and increased ERK1/2 phosphorylation in the hippocampus in a dose-dependent manner. These results demonstrate that CPT facilitates neurite outgrowth and enhances learning and memory, which may be mediated by facilitating ERK1/2 signal.
Journal
Food Chem Toxicol
Publish Year
2020
Experiment Subject
mouse; mouse neuroblastoma cell line; neuro2a cell line
Experiment Type
Cell Experiment
Phenotype Related
Neuroblastoma
Paper Title Cn
Paper Title En
Cryptotanshinone enhances neurite outgrowth and memory via extracellular signal-regulated kinase 1/2 signaling
Bilingual Status
semi_complete