ReferenceID 4965
Isobavachalcone inhibits acute myeloid leukemia: Potential role for ROS-dependent mitochondrial apoptosis and differentiation
Phytother Res
Isobavachalcone (IBC) has been shown to induce apoptosis and differentiation of acute myeloid leukemia (AML) cells. However, the underlying molecular mechanisms are not fully understood. Herein, IBC exhibited significant
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Record Fields
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- Reference Id
- 4965
- Evidence Id
- 21555
- Core Evidence Id
- 21555
- Source Reference Id
- 3179
- Herb2 Reference Id
- HBREF003976
- Subject Paper Key
- HBIN021558_33624885
- Pubmed Id
- 33624885
- Doi
- 10.1002/ptr.7054
- Paper Title
- Isobavachalcone inhibits acute myeloid leukemia: Potential role for ROS-dependent mitochondrial apoptosis and differentiation
- Paper Abstract
- Isobavachalcone (IBC) has been shown to induce apoptosis and differentiation of acute myeloid leukemia (AML) cells. However, the underlying molecular mechanisms are not fully understood. Herein, IBC exhibited significant inhibition on the cell viability, proliferation, and the colony formation ability of AML cells. Moreover, IBC induced mitochondrial apoptosis evidenced by reduced mitochondrial membrane potential, increased Bax level, decreased Bcl-2, Bcl-xL, and Mcl-1 levels, elevated cytochrome c level in the cytosol and increased cleavage of caspase-9, caspase-3, and PARP. Furthermore, IBC obviously promoted the differentiation of AML cells, accompanied by the increase of the phosphorylation of MEK and ERK and the C/EBPalpha expression as well as the C/EBPbeta LAP/LIP isoform ratio, which was significantly reversed by U0126, a specific inhibitor of MEK. Notably, IBC enhanced the intracellular ROS level. More importantly, IBC-induced apoptosis and differentiation of HL-60 cells were significantly mitigated by NAC. In addition, IBC also exhibited an obvious anti-AML effect in NOD/SCID mice with the engraftment of HL-60 cells. Together, our study suggests that the ROS-medicated signaling pathway is highly involved in IBC-induced apoptosis and differentiation of AML cells.
- Journal
- Phytother Res
- Publish Year
- 2021
- Experiment Subject
- mouse; hl-60 cells
- Experiment Type
- Animal Experiment
- Phenotype Related
- Acute Myeloid Leukemia
- Paper Title Cn
- Paper Title En
- Isobavachalcone inhibits acute myeloid leukemia: Potential role for ROS-dependent mitochondrial apoptosis and differentiation
- Bilingual Status
- semi_complete