ReferenceID 4955
Efficacy and mechanism study of cordycepin against brain metastases of small cell lung cancer based on zebrafish
Phytomedicine
Background: Small cell lung cancer (SCLC) is an aggressive tumor with high brain metastasis (BM) potential. There has been no significant progress in the treatment of SCLC for more than 30 years. Cordycepin has shown the
Relationship Network
Interactive first-hop connections across herbs, ingredients, formulas, targets, diseases, symptoms, syndromes, evidence, and monographs.
Click a node to open it in a new tab
Ingredient: 1Reference: 1Links: 1
Arranging relationship network...
Record Fields
Scalar fields from the final reference record.
- Reference Id
- 4955
- Evidence Id
- 21545
- Core Evidence Id
- 21545
- Source Reference Id
- 3163
- Herb2 Reference Id
- HBREF003960
- Subject Paper Key
- HBIN021460_36610112
- Pubmed Id
- 36610112
- Doi
- 10.1016/j.phymed.2022.154613
- Paper Title
- Efficacy and mechanism study of cordycepin against brain metastases of small cell lung cancer based on zebrafish
- Paper Abstract
- Background: Small cell lung cancer (SCLC) is an aggressive tumor with high brain metastasis (BM) potential. There has been no significant progress in the treatment of SCLC for more than 30 years. Cordycepin has shown the therapeutic potential for cancer by modulating multiple cellular signaling pathways. However, the effect and mechanism of cordycepin on anti-SCLC BM remain unknown. Purpose: In this study, we focused on the anti-SCLC BM effect of cordycepin in the zebrafish model and its potential mechanism. Study design and methods: A SCLC xenograft model based on zebrafish embryos and in vitro cell migration assay were established. Cordycepin was administrated by soaking and microinjection in the zebrafish model. RNA-seq assay was performed to analyze transcriptomes of different groups. Geno Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment were performed to reveal the underlying mechanism. Real-time qPCR was used to verify the effects of cordycepin on the key genes. Results: Cordycepin showed lower cytotoxicity in vitro compared with cisplatin, anlotinib and etoposide, but showed comparable anti-proliferation and anti-BM effects in zebrafish SCLC xenograft model. Cordycepin showed significant anti-SCLC BM effects when administrated by both soaking and microinjection. RNA-seq demonstrated that cordycepin was involved in vitamin D metabolism, lipid transport, and proteolysis in cellular protein catabolic process pathways in SCLC BM microenvironment in zebrafish, and was involved in regulating the expressions of key genes such as cyp24a1, apoa1a, ctsl. The anti-BM effect of cordycepin in SCLC was mediated by reversing the expression of these genes. Conclusion: Our work is the first to describe the mechanism of cordycepin against SCLC BM from the perspective of regulating the brain microenvironment, providing new evidence for the anti-tumor effect of cordycepin.
- Journal
- Phytomedicine
- Publish Year
- 2023
- Experiment Subject
- zebrafish
- Experiment Type
- Animal Experiment
- Phenotype Related
- Small Cell Lung Cancer; Anti-sclc; Tumor; Cancer; High Brain Metastasis
- Paper Title Cn
- Paper Title En
- Efficacy and mechanism study of cordycepin against brain metastases of small cell lung cancer based on zebrafish
- Bilingual Status
- semi_complete