ReferenceID 4900
Chrysin Inhibits TNFα-Induced TSLP Expression through Downregulation of EGR1 Expression in Keratinocytes
Int J Mol Sci
Thymic stromal lymphopoietin (TSLP) is an epithelial cell-derived cytokine that acts as a critical mediator in the pathogenesis of atopic dermatitis (AD). Various therapeutic agents that prevent TSLP function can efficie
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Record Fields
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- Reference Id
- 4900
- Evidence Id
- 21490
- Core Evidence Id
- 21490
- Source Reference Id
- 3053
- Herb2 Reference Id
- HBREF003850
- Subject Paper Key
- HBIN020447_33919431
- Pubmed Id
- 33919431
- Doi
- 10.3390/ijms22094350
- Paper Title
- Chrysin Inhibits TNFα-Induced TSLP Expression through Downregulation of EGR1 Expression in Keratinocytes
- Paper Abstract
- Thymic stromal lymphopoietin (TSLP) is an epithelial cell-derived cytokine that acts as a critical mediator in the pathogenesis of atopic dermatitis (AD). Various therapeutic agents that prevent TSLP function can efficiently relieve the clinical symptoms of AD. However, the downregulation of TSLP expression by therapeutic agents remains poorly understood. In this study, we investigated the mode of action of chrysin in TSLP suppression in an AD-like inflammatory environment. We observed that the transcription factor early growth response (EGR1) contributed to the tumor necrosis factor alpha (TNFalpha)-induced transcription of TSLP. Chrysin attenuated TNFalpha-induced TSLP expression by downregulating EGR1 expression in HaCaT keratinocytes. We also showed that the oral administration of chrysin improved AD-like skin lesions in the ear and neck of BALB/c mice challenged with 2,4-dinitrochlorobenzene. We also showed that chrysin suppressed the expression of EGR1 and TSLP by inhibiting the extracellular signal-regulated kinase (ERK) 1/2 and c-Jun N-terminal kinase (JNK) 1/2 mitogen-activated protein kinase pathways. Collectively, the findings of this study suggest that chrysin improves AD-like skin lesions, at least in part, through the downregulation of the ERK1/2 or JNK1/2-EGR1-TSLP signaling axis in keratinocytes.
- Journal
- Int J Mol Sci
- Publish Year
- 2021
- Experiment Subject
- mouse; hacat keratinocytes
- Experiment Type
- Animal Experiment
- Phenotype Related
- Ad-like Skin Lesions; Tumor; Atopic Dermatitis; Skin Lesions
- Paper Title Cn
- Paper Title En
- Chrysin Inhibits TNFα-Induced TSLP Expression through Downregulation of EGR1 Expression in Keratinocytes
- Bilingual Status
- semi_complete