ReferenceID 4875
Castanospermine suppresses CD44 ectodomain cleavage as revealed by transmembrane bioluminescent sensors
J Cell Sci
Cluster of differentiation 44 (CD44) is a single-pass transmembrane glycoprotein that is a widely distributed cell-surface adhesion molecule. CD44 undergoes ectodomain cleavage by membrane-associated metalloproteinases i
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Record Fields
Scalar fields from the final reference record.
- Reference Id
- 4875
- Evidence Id
- 21465
- Core Evidence Id
- 21465
- Source Reference Id
- 2993
- Herb2 Reference Id
- HBREF003790
- Subject Paper Key
- HBIN019897_35194645
- Pubmed Id
- 35194645
- Doi
- 10.1242/jcs.259314
- Paper Title
- Castanospermine suppresses CD44 ectodomain cleavage as revealed by transmembrane bioluminescent sensors
- Paper Abstract
- Cluster of differentiation 44 (CD44) is a single-pass transmembrane glycoprotein that is a widely distributed cell-surface adhesion molecule. CD44 undergoes ectodomain cleavage by membrane-associated metalloproteinases in breast cancer cells. Cleavage plays a critical role in cancer cell migration by mediating the interaction between CD44 and the extracellular matrix. To explore inhibitors of CD44 ectodomain cleavage, we developed two bioluminescent sensors for the detection of CD44 ectodomain cleavage. The sensors were designed as two-transmembrane proteins with split-luciferase fragments, one of which was cyclized by protein trans-splicing of a DnaE intein. These two sensors emit light by the cyclization or the spontaneous complementation of the luciferase fragments. The luminescence intensities decreased upon cleavage of the ectodomain in breast cancer cells. The sensors revealed that castanospermine, an α-glucosidase inhibitor, suppressed the ectodomain cleavage of endogenous CD44 in breast cancer cells. Castanospermine also inhibited breast cancer cell invasion. Thus, the sensors are beneficial tools for evaluating the effects of different inhibitors.
- Journal
- J Cell Sci
- Publish Year
- 2022
- Experiment Subject
- Experiment Type
- Cell Experiment
- Phenotype Related
- Cancer; Breast Cancer
- Paper Title Cn
- Paper Title En
- Castanospermine suppresses CD44 ectodomain cleavage as revealed by transmembrane bioluminescent sensors
- Bilingual Status
- semi_complete