ReferenceID 4849

Capsaicin Inhibits Shigella flexneri Intracellular Growth by Inducing Autophagy

Front Pharmacol

Antibiotic treatment plays an essential role in preventing Shigella infection. However, incidences of global rise in antibiotic resistance create a major challenge to treat bacterial infection. In this context, there is

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Reference Id
4849
Evidence Id
21439
Core Evidence Id
21439
Source Reference Id
2948
Herb2 Reference Id
HBREF003745
Subject Paper Key
HBIN019690_35873583
Pubmed Id
35873583
Doi
10.3389/fphar.2022.903438
Paper Title
Capsaicin Inhibits Shigella flexneri Intracellular Growth by Inducing Autophagy
Paper Abstract
Antibiotic treatment plays an essential role in preventing Shigella infection. However, incidences of global rise in antibiotic resistance create a major challenge to treat bacterial infection. In this context, there is an urgent need for newer approaches to reduce S. flexneri burden. This study largely focuses on the role of the herbal compound capsaicin (Caps) in inhibiting S. flexneri growth and evaluating the molecular mechanism behind bacterial clearance. Here, we show for the first time that Caps inhibits intracellular S. flexneri growth by inducing autophagy. Activation of autophagy by Caps is mediated through transcription factor TFEB, a master regulator of autophagosome biogenesis. Caps induced the nuclear localization of TFEB. Activation of TFEB further induces the gene transcription of autophagosomal genes. Our findings revealed that the inhibition of autophagy by silencing TFEB and Atg5 induces bacterial growth. Hence, Caps-induced autophagy is one of the key factors responsible for bacterial clearance. Moreover, Caps restricted the intracellular proliferation of S. flexneri -resistant strain. The efficacy of Caps in reducing S. flexneri growth was confirmed by an animal model. This study showed for the first time that S. flexneri infection can be inhibited by inducing autophagy. Overall observations suggest that Caps activates TFEB to induce autophagy and thereby combat S. flexneri infection.
Journal
Front Pharmacol
Publish Year
2022
Experiment Subject
Experiment Type
Cell Experiment
Phenotype Related
Bacterial Infection; Shigella Infection
Paper Title Cn
Paper Title En
Capsaicin Inhibits Shigella flexneri Intracellular Growth by Inducing Autophagy
Bilingual Status
semi_complete