ReferenceID 4782
Calycosin, a Common Dietary Isoflavonoid, Suppresses Melanogenesis through the Downregulation of PKA/CREB and p38 MAPK Signaling Pathways
Int J Mol Sci
Calycosin, a bioactive isoflavonoid isolated from root extracts of Astragalus membranaceus , has been reported to inhibit melanogenesis, the mechanism of which remains undefined. In this study, we interrogated the mechan
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Record Fields
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- Reference Id
- 4782
- Evidence Id
- 21372
- Core Evidence Id
- 21372
- Source Reference Id
- 2805
- Herb2 Reference Id
- HBREF003602
- Subject Paper Key
- HBIN019425_35163281
- Pubmed Id
- 35163281
- Doi
- 10.3390/ijms23031358
- Paper Title
- Calycosin, a Common Dietary Isoflavonoid, Suppresses Melanogenesis through the Downregulation of PKA/CREB and p38 MAPK Signaling Pathways
- Paper Abstract
- Calycosin, a bioactive isoflavonoid isolated from root extracts of Astragalus membranaceus , has been reported to inhibit melanogenesis, the mechanism of which remains undefined. In this study, we interrogated the mechanistic basis by which calycosin inhibits melanin production in two model systems, i.e., B16F10 melanoma cells and zebrafish embryos. Calycosin was effective in protecting B16F10 cells from α-melanocyte-stimulating hormone (α-MSH)-induced melanogenesis and tyrosinase activity. This anti-melanogenic effect was accompanied by decreased expression levels of microphthalmia-associated transcription factor (MITF), a key protein controlling melanin synthesis, and its target genes tyrosinase and tyrosinase-related protein-2 (TRP-2) in calycosin-treated cells. Mechanistically, we obtained the first evidence that calycosin-mediated MITF downregulation was attributable to its ability to block signaling pathways mediated by cAMP response element-binding protein (CREB) and p38 MAP kinase. The protein kinase A (PKA) inhibitor H-89 and p38 inhibitor SB203580 validated the premise that calycosin inhibits melanin synthesis and tyrosinase activity by regulating the PKA/CREB and p38 MAPK signaling pathways. Moreover, the in vivo anti-melanogenic efficacy of calycosin was manifested by its ability to suppress body pigmentation and tyrosinase activity in zebrafish embryos. Together, these data suggested the translational potential of calycosin to be developed as skin-lightening cosmeceuticals.
- Journal
- Int J Mol Sci
- Publish Year
- 2022
- Experiment Subject
- b16f10 cells; b16f10 melanoma cells; calycosin-treated cells; zebrafish
- Experiment Type
- Animal & Cell Experiment
- Phenotype Related
- Melanoma
- Paper Title Cn
- Paper Title En
- Calycosin, a Common Dietary Isoflavonoid, Suppresses Melanogenesis through the Downregulation of PKA/CREB and p38 MAPK Signaling Pathways
- Bilingual Status
- semi_complete