ReferenceID 4735
Overexpression of ABCC1 Confers Drug Resistance to Betulin
Front Oncol
Betulin is a lupane-type pentacyclic triterpene, which is isolated from birch bark. It has a broad spectrum of biological and pharmacological properties, such as anti-inflammatory, anti-tumor, anti-viral, and anti-bacter
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Record Fields
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- Reference Id
- 4735
- Evidence Id
- 21325
- Core Evidence Id
- 21325
- Source Reference Id
- 2712
- Herb2 Reference Id
- HBREF003509
- Subject Paper Key
- HBIN018374_33718236
- Pubmed Id
- 33718236
- Doi
- 10.3389/fonc.2021.640656
- Paper Title
- Overexpression of ABCC1 Confers Drug Resistance to Betulin
- Paper Abstract
- Betulin is a lupane-type pentacyclic triterpene, which is isolated from birch bark. It has a broad spectrum of biological and pharmacological properties, such as anti-inflammatory, anti-tumor, anti-viral, and anti-bacterial activity. Herein, we explored the factors that may result in betulin resistance, especially with respect to its interaction with ATP-binding cassette subfamily C member 1 (ABCC1). ABCC1 is an important member of the ATP-binding cassette (ABC) transporter family, which is central to mediating multidrug resistance (MDR) in naturally derived anticancer agents. An MTT-based cell viability assay showed that ABCC1 overexpression has the ability to desensitize both cancer cell line and gene-transfected cell line to betulin and that this betulin-induced resistance can be antagonized by a known ABCC1 inhibitor MK571 at 25 muM. Additionally, betulin upregulates the ABCC1 protein expression level in both concentration-dependent and time-dependent manners, also blocks the transport function mediated by ABCC1. Subsequently, a high affinity score of betulin was achieved in a computational docking analysis, demonstrating a strong interaction of betulin with ABCC1.
- Journal
- Front Oncol
- Publish Year
- 2021
- Experiment Subject
- Experiment Type
- Cell Experiment
- Phenotype Related
- Cancer
- Paper Title Cn
- Paper Title En
- Overexpression of ABCC1 Confers Drug Resistance to Betulin
- Bilingual Status
- semi_complete