ReferenceID 4659
Berbamine hydrochloride inhibits bovine viral diarrhea virus replication via interfering in late-stage autophagy
Virus Res
Bovine viral diarrhea virus (BVDV) is a harmful pathogen that easily causes large-scale infections and huge economic losses to the cattle industry. Berbamine hydrochloride (BBH) is a natural product extracted from berber
Relationship Network
Interactive first-hop connections across herbs, ingredients, formulas, targets, diseases, symptoms, syndromes, evidence, and monographs.
Click a node to open it in a new tab
Ingredient: 1Reference: 1Links: 1
Arranging relationship network...
Record Fields
Scalar fields from the final reference record.
- Reference Id
- 4659
- Evidence Id
- 21249
- Core Evidence Id
- 21249
- Source Reference Id
- 2582
- Herb2 Reference Id
- HBREF003379
- Subject Paper Key
- HBIN017888_36064041
- Pubmed Id
- 36064041
- Doi
- 10.1016/j.virusres.2022.198905
- Paper Title
- Berbamine hydrochloride inhibits bovine viral diarrhea virus replication via interfering in late-stage autophagy
- Paper Abstract
- Bovine viral diarrhea virus (BVDV) is a harmful pathogen that easily causes large-scale infections and huge economic losses to the cattle industry. Berbamine hydrochloride (BBH) is a natural product extracted from berberis and has a wide range of pharmacological effects. However, the antiviral effect of BBH against BVDV needs to be further elucidated. This study aimed to evaluate the antiviral activities of BBH against BVDV infection. We mainly used RT-qPCR, Western blotting, immunofluorescence, and TEM assays to assess the inhibitory activity of BBH against BVDV. The results showed that BBH had an inhibitory effect on BVDV and higher inhibitory activity in the viral attachment and release in MDBK cells. This study found that BVDV could induce and use autophagy to replicate itself. Further results showed that BBH inhibited BVDV infection by inhibiting autophagy integrity in BVDV-infected cells, which was proven by the detection of autophagy-related proteins. Our data show that in BBH-treated BVDV-infected cells, the expression of p62 and LC3 increased over time. After the addition of an autophagy inhibitor, chloroquine (CQ), and an autophagy promoter, rapamycin (Rapa), we found that promoting autophagy was beneficial to the replication of BVDV, while inhibiting autophagy could reduce the number of infections by BVDV, which was evidenced by the expression of the BVDV E2 protein. Furthermore, BBH blocked the normal binding of LC3 and LAMP1 in BVDV-infected cells. In conclusion, BBH inhibited BVDV infection by inhibiting BVDV-induced autophagy in cells, and its inhibitory effect was obvious in the viral attachment and release stages. Therefore, our study provides a new idea for exploring novel anti-BVDV drugs.
- Journal
- Virus Res
- Publish Year
- 2022
- Experiment Subject
- bbh-treated bvdv-infected cells; cattle; mdbk cells
- Experiment Type
- Cell Experiment
- Phenotype Related
- Bovine Viral Diarrhea Virus
- Paper Title Cn
- Paper Title En
- Berbamine hydrochloride inhibits bovine viral diarrhea virus replication via interfering in late-stage autophagy
- Bilingual Status
- semi_complete