ReferenceID 4621
Avicularin suppresses cartilage extracellular matrix degradation and inflammation via TRAF6/MAPK activation
Phytomedicine
BACKGROUND: Osteoarthritis (OA) is an intractable degenerative disease of the whole joint, which is characterized by synovitis inflammation, cartilage damage, and chronic pain. Tumor necrosis factor receptor (TNFR)-assoc
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- Reference Id
- 4621
- Evidence Id
- 21211
- Core Evidence Id
- 21211
- Source Reference Id
- 2509
- Herb2 Reference Id
- HBREF003306
- Subject Paper Key
- HBIN017408_34371251
- Pubmed Id
- 34371251
- Doi
- 10.1016/j.phymed.2021.153657
- Paper Title
- Avicularin suppresses cartilage extracellular matrix degradation and inflammation via TRAF6/MAPK activation
- Paper Abstract
- BACKGROUND: Osteoarthritis (OA) is an intractable degenerative disease of the whole joint, which is characterized by synovitis inflammation, cartilage damage, and chronic pain. Tumor necrosis factor receptor (TNFR)-associated factor 6 (TRAF6) performs an important role in OA. PURPOSE: We aim to investigate avicularin to protect cartilage extracellular matrix degradation (ECM) and suppresses inflammation both in rat and human chondrocytes. METHODS: 5-Ethynyl-2'-deoxyuridine (EdU) staining, Quantitative real-time PCR, TRAF6 plasmid transfection, Western blot, Measurement of nitric oxide (NO), ROS detection and Immunofluorescence were utilized in vitro. micro-CT scanning, Safranin O-Fast Green, toluidine blue and immunohistochemistry staining were performed in vivo. RESULTS: In vitro, avicularin attenuates the degradation of ECM and inflammation, which could inhibit the activation of TRAF6/MAPK pathway via targeting TRAF6. Increased MMP3 and MMP13 expressions and decreased Aggrecan and Collagen II levels were observed in anterior cruciate ligament transection (ACLT) induced osteoarthritic rats. Interestingly, intra-articular injection of avicularin attenuates this phenomenon. CONCLUSIONS: Taken together, our results indicate that avicularin suppresses cartilage extracellular matrix degradation and inflammation via TRAF6/MAPK activation by targeting TRAF6. These observations identify TRAF6 as a relevant drug target, and avicularin may as a potential therapeutic agent in osteoarthritis.
- Journal
- Phytomedicine
- Publish Year
- 2021
- Experiment Subject
- rat; human
- Experiment Type
- Cell Experiment
- Phenotype Related
- Synovitis Inflammation; Cartilage Damage; Osteoarthritis; Anterior Cruciate Ligament Transection; Chronic Pain; Intractable Degenerative Disease Of The Whole Joint; Osteoarthritic
- Paper Title Cn
- Paper Title En
- Avicularin suppresses cartilage extracellular matrix degradation and inflammation via TRAF6/MAPK activation
- Bilingual Status
- semi_complete