ReferenceID 4617
Atractylodin Produces Antinociceptive Effect through a Long-Lasting TRPA1 Channel Activation
Int J Mol Sci
Atractylodin (ATR) is a bioactive component found in dried rhizomes of Atractylodes lancea (AL) De Candolle. Although AL has accumulated empirical evidence for the treatment of pain, the molecular mechanism underlying th
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Record Fields
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- Reference Id
- 4617
- Evidence Id
- 21207
- Core Evidence Id
- 21207
- Source Reference Id
- 2499
- Herb2 Reference Id
- HBREF003296
- Subject Paper Key
- HBIN017294_33807167
- Pubmed Id
- 33807167
- Doi
- 10.3390/ijms22073614
- Paper Title
- Atractylodin Produces Antinociceptive Effect through a Long-Lasting TRPA1 Channel Activation
- Paper Abstract
- Atractylodin (ATR) is a bioactive component found in dried rhizomes of Atractylodes lancea (AL) De Candolle. Although AL has accumulated empirical evidence for the treatment of pain, the molecular mechanism underlying the anti-pain effect of ATR remains unclear. In this study, we found that ATR increases transient receptor potential ankyrin-1 (TRPA1) single-channel activity in hTRPA1 expressing HEK293 cells. A bath application of ATR produced a long-lasting calcium response, and the response was completely diminished in the dorsal root ganglion neurons of TRPA1 knockout mice. Intraplantar injection of ATR evoked moderate and prolonged nociceptive behavior compared to the injection of allyl isothiocyanate (AITC). Systemic application of ATR inhibited AITC-induced nociceptive responses in a dose-dependent manner. Co-application of ATR and QX-314 increased the noxious heat threshold compared with AITC in vivo. Collectively, we concluded that ATR is a unique agonist of TRPA1 channels, which produces long-lasting channel activation. Our results indicated ATR-mediated anti-nociceptive effect through the desensitization of TRPA1-expressing nociceptors.
- Journal
- Int J Mol Sci
- Publish Year
- 2021
- Experiment Subject
- mouse; htrpa1 expressing hek293 cells
- Experiment Type
- Animal Experiment
- Phenotype Related
- Paper Title Cn
- Paper Title En
- Atractylodin Produces Antinociceptive Effect through a Long-Lasting TRPA1 Channel Activation
- Bilingual Status
- semi_complete