ReferenceID 4523
Asiaticoside inhibits TGF-β1-induced mesothelial-mesenchymal transition and oxidative stress via the Nrf2/HO-1 signaling pathway in the human peritoneal mesothelial cell line HMrSV5
Cell Mol Biol Lett
Background: Peritoneal fibrosis (PF) is a frequent complication caused by peritoneal dialysis (PD). Peritoneal mesothelial cells (PMCs), the first barrier of the peritoneum, play an important role in maintaining structur
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Record Fields
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- Reference Id
- 4523
- Evidence Id
- 21113
- Core Evidence Id
- 21113
- Source Reference Id
- 2442
- Herb2 Reference Id
- HBREF003239
- Subject Paper Key
- HBIN017061_32514269
- Pubmed Id
- 32514269
- Doi
- 10.1186/s11658-020-00226-9
- Paper Title
- Asiaticoside inhibits TGF-β1-induced mesothelial-mesenchymal transition and oxidative stress via the Nrf2/HO-1 signaling pathway in the human peritoneal mesothelial cell line HMrSV5
- Paper Abstract
- Background: Peritoneal fibrosis (PF) is a frequent complication caused by peritoneal dialysis (PD). Peritoneal mesothelial cells (PMCs), the first barrier of the peritoneum, play an important role in maintaining structure and function in the peritoneum during PD. Mesothelial-mesenchymal transition (MMT) and oxidative stress of PMCs are two key processes of PF. Purpose: To elucidate the efficacy and possible mechanism of asiaticoside inhibition of MMT and ROS generation in TGF-beta1-induced PF in human peritoneal mesothelial cells (HPMCs). Methods: MMT and ROS generation of HPMCs were induced by TGF-beta1. To explain the anti-MMT and antioxidant role of asiaticoside, varied doses of asiaticoside, oxygen radical scavenger (NAC), TGF-beta receptor kinase inhibitor (LY2109761) and Nrf2 inhibitor (ML385) were used separately. Immunoblots were used to detect the expression of signaling associated proteins. DCFH-DA was used to detect the generation of ROS. Transwell migration assay and wound healing assay were used to verify the capacity of asiaticoside to inhibit MMT. Immunofluorescence assay was performed to observe the subcellular translocation of Nrf2 and expression of HO-1. Results: Asiaticoside inhibited TGF-beta1-induced MMT and suppressed Smad signaling in a dose-dependent manner. Migration and invasion activities of HPMCs were decreased by asiaticoside. Asiaticoside decreased TGF-beta1-induced ROS, especially in a high dose (150 muM) for 6 h. Furthermore, ML385 partly abolished the inhibitory effect of asiaticoside on MMT, ROS and p-Smad2/3. Conclusions: Asiaticoside inhibited the TGF-beta1-induced MMT and ROS via Nrf2 activation, thus protecting the peritoneal membrane and preventing PF.
- Journal
- Cell Mol Biol Lett
- Publish Year
- 2020
- Experiment Subject
- human
- Experiment Type
- Cell Experiment
- Phenotype Related
- Peritoneal Fibrosis
- Paper Title Cn
- Paper Title En
- Asiaticoside inhibits TGF-β1-induced mesothelial-mesenchymal transition and oxidative stress via the Nrf2/HO-1 signaling pathway in the human peritoneal mesothelial cell line HMrSV5
- Bilingual Status
- semi_complete