ReferenceID 4495
Effects of Andrographolide on Intracellular pH Regulation, Cellular Migration, and Apoptosis in Human Cervical Cancer Cells †
Cancers (Basel)
Cancer cells have been characterized with alkaline intracellular pH (pHi) values (>=7.2) to enable cancer proliferation, migration, and progression. The aim of the present study was to explore the concentration-dependent
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Record Fields
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- Reference Id
- 4495
- Evidence Id
- 21085
- Core Evidence Id
- 21085
- Source Reference Id
- 2267
- Herb2 Reference Id
- HBREF003064
- Subject Paper Key
- HBIN016019_32046125
- Pubmed Id
- 32046125
- Doi
- 10.3390/cancers12020387
- Paper Title
- Effects of Andrographolide on Intracellular pH Regulation, Cellular Migration, and Apoptosis in Human Cervical Cancer Cells †
- Paper Abstract
- Cancer cells have been characterized with alkaline intracellular pH (pHi) values (>=7.2) to enable cancer proliferation, migration, and progression. The aim of the present study was to explore the concentration-dependent effects of Andrographolide, an active diterpenoid compound of herb Andrographis paniculata, on Na+/H+ exchanger isoform 1 (NHE1), cellular migration and apoptosis in human cervical cancer cells (HeLa). The pHi was detected by microspectrofluorometry method, and intracellular acidification was induced by NH4Cl prepulse technique. Viability and protein expression were determined by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and Western blot, respectively. Human normal endocervical cells (End1), ectocervical cells (Ect1), and HeLa were bought commercially. The resting pHi value of HeLa ( 7.47) was significantly higher than that of End1 and Ect1 ( 7.30), and shifted from alkaline to acidic following acid/base impacts. In HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) -buffered superfusate, NHE1 and V-ATPase co-existed functionally for acid extrusion in HeLa, while only NHE1 existed functionally in End/Ect1. Andrographolide (3-1000 muM) concentration-dependently inhibited NHE1 activity. Cell-migration and expressions of NHE1, V-ATPase, PARP (poly-ADP-ribose-polymerase), pro-Caspase-3, and Bcl-2 were significantly reduced by pretreating with Andrographolide (>=100 muM) for 24-48 h in HeLa. Andrographolide inhibited cell viability of End1-cells/Ect1 and HeLa (>=100 and >=30 muM, respectively). The present findings implicate the promising clinical applications of Andrographolide on cervical cancer treatment.
- Journal
- Cancers (Basel)
- Publish Year
- 2020
- Experiment Subject
- human; ect1; end1; hela
- Experiment Type
- Cell Experiment
- Phenotype Related
- Cancer; Cervical Cancer
- Paper Title Cn
- Paper Title En
- Effects of Andrographolide on Intracellular pH Regulation, Cellular Migration, and Apoptosis in Human Cervical Cancer Cells †
- Bilingual Status
- semi_complete