ReferenceID 4494
Andrographolide promotes pancreatic duct cells differentiation into insulin-producing cells by targeting PDX-1
Biochem Pharmacol
Regeneration of beta-cells by differentiation of pancreatic progenitor cells has the potential to fundamentally solve the problems of the loss of beta-cell function and mass during disease progression in both type 1 or 2
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Record Fields
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- Reference Id
- 4494
- Evidence Id
- 21084
- Core Evidence Id
- 21084
- Source Reference Id
- 2266
- Herb2 Reference Id
- HBREF003063
- Subject Paper Key
- HBIN016019_31887289
- Pubmed Id
- 31887289
- Doi
- 10.1016/j.bcp.2019.113785
- Paper Title
- Andrographolide promotes pancreatic duct cells differentiation into insulin-producing cells by targeting PDX-1
- Paper Abstract
- Regeneration of beta-cells by differentiation of pancreatic progenitor cells has the potential to fundamentally solve the problems of the loss of beta-cell function and mass during disease progression in both type 1 or 2 diabetes. Therefore, discovery of novel differentiation inducers to promote islet regeneration is of great significance. Pancreatic and duodenal homeobox1 (PDX-1) is a key transcription factor that promotes the development and maturation of pancreatic beta-cells. To screen potential novel small molecules for enhancing differentiation of PNAC-1 cells, a human pancreatic ductal cell lines into insulin-producing cells (IPCs), we developed a high-throughput screening method through fusing the PDX-1 promoter region with a luciferase reporter gene. We screened and identified that andrographolide named C1037 stimulates PDX-1 expression in both mRNA and protein level and significantly promotes PANC-1 cells differentiation into IPCs as compared with that of control cells. The therapeutic effect of C037 in Streptozotocin induced diabetic mouse model through differentiation of pancreatic ductal cells into insulin positive islets was also observed. Our study provides a novel method to screen compounds regulating the differentiation of pancreatic progenitor cells having the potential of enhancing islet regeneration for diabetes therapy.
- Journal
- Biochem Pharmacol
- Publish Year
- 2020
- Experiment Subject
- mouse; human; human pancreatic ductal cell lines; panc-1 cells; pnac-1 cells
- Experiment Type
- Cell Experiment
- Phenotype Related
- Diabetes; Diabetic; Type 1 Or 2 Diabetes
- Paper Title Cn
- Paper Title En
- Andrographolide promotes pancreatic duct cells differentiation into insulin-producing cells by targeting PDX-1
- Bilingual Status
- semi_complete