ReferenceID 4492
Amygdalin Attenuates Atherosclerosis and Plays an Anti-Inflammatory Role in ApoE Knock-Out Mice and Bone Marrow-Derived Macrophages
Front Pharmacol
Amygdalin, the main component of Prunus persica (L.) Stokes, has been used to treat atherosclerosis in mouse model due to its anti-inflammatory role. However, the underlying mechanism remains poorly understood. This stud
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Record Fields
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- Reference Id
- 4492
- Evidence Id
- 21082
- Core Evidence Id
- 21082
- Source Reference Id
- 2262
- Herb2 Reference Id
- HBREF003059
- Subject Paper Key
- HBIN015934_33192531
- Pubmed Id
- 33192531
- Doi
- 10.3389/fphar.2020.590929
- Paper Title
- Amygdalin Attenuates Atherosclerosis and Plays an Anti-Inflammatory Role in ApoE Knock-Out Mice and Bone Marrow-Derived Macrophages
- Paper Abstract
- Amygdalin, the main component of Prunus persica (L.) Stokes, has been used to treat atherosclerosis in mouse model due to its anti-inflammatory role. However, the underlying mechanism remains poorly understood. This study aimed to evidence the influence of amygdalin on high-fat diet-induced atherosclerosis in ApoE knock-out (ApoE-/-) mice, and unravel its anti-inflammatory mechanism. ApoE-/- mice fed with high-fat diet for eight weeks were randomly divided into four groups and injected with amygdalin at the concentration of 0.08 or 0.04 mg/kg for 12 weeks. Additionally, bone marrow-derived macrophages were intervened with oxidized low-density lipoprotein (oxLDL) or lipopolysaccharide plus various concentrations of amygdalin for further exploration. Body weight, serum lipid profiles and inflammatory cytokines were detected by ELISA, gene expression by RT-PCR, plaque sizes by Oil Red O, lymphatic vessels of heart atrium and Tnfalpha production by immunofluorescence staining. MAPKs, AP-1 and NF-kappaB p65 pathways were also explored. Amygdalin decreased body weight, serum lipids, plaque size, lymphatic vessels and inflammatory cytokines (Il-6, Tnfalpha), Nos1 and Nos2, and increased Il-10 expression in ApoE-/- mice. In oxLDL-induced bone marrow-derived macrophages, amygdalin reduced inflammatory cytokines (Il-6, Tnfalpha), Nos1 and Nos2, and increased Il-10 production. These effects were associated with the decreased phosphorylation of Mapk1, Mapk8, Mapk14, Fos and Jun, and the translocation of NF-kappaB p65 from nucleus to cytoplasm. The results suggested that amygdalin could attenuate atherosclerosis and play an anti-inflammatory role via MAPKs, AP-1 and NF-kappaB p65 signaling pathways in ApoE-/- mice and oxLDL-treated bone marrow-derived macrophages.
- Journal
- Front Pharmacol
- Publish Year
- 2020
- Experiment Subject
- mouse; oxldl-treated bone marrow-derived macrophages
- Experiment Type
- Animal Experiment
- Phenotype Related
- Atherosclerosis
- Paper Title Cn
- Paper Title En
- Amygdalin Attenuates Atherosclerosis and Plays an Anti-Inflammatory Role in ApoE Knock-Out Mice and Bone Marrow-Derived Macrophages
- Bilingual Status
- semi_complete