ReferenceID 4477
Amentoflavone inhibits tumor necrosis factor-α-induced migration and invasion through AKT/mTOR/S6k1/hedgehog signaling in human breast cancer
Food Funct
Inflammatory cytokine tumor necrosis factor-alpha (TNFalpha) has been demonstrated to accelerate the progression and metastasis of various carcinomas. In this study, we investigated the effect of amentoflavone on inhibit
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Record Fields
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- Reference Id
- 4477
- Evidence Id
- 21067
- Core Evidence Id
- 21067
- Source Reference Id
- 2236
- Herb2 Reference Id
- HBREF003033
- Subject Paper Key
- HBIN015834_34542136
- Pubmed Id
- 34542136
- Doi
- 10.1039/d1fo01085a
- Paper Title
- Amentoflavone inhibits tumor necrosis factor-α-induced migration and invasion through AKT/mTOR/S6k1/hedgehog signaling in human breast cancer
- Paper Abstract
- Inflammatory cytokine tumor necrosis factor-alpha (TNFalpha) has been demonstrated to accelerate the progression and metastasis of various carcinomas. In this study, we investigated the effect of amentoflavone on inhibiting the migration and invasion of TNFalpha-induced breast cancer cells. Results showed that amentoflavone significantly blocked the cellular migration and invasion of MCF10DCIS.com and MDA-MB-231 cells at a concentration of 10 muM but did not affect the cell viability. The mRNA and protein levels of matrix metalloproteinase (MMP)-9, significantly activated by TNFalpha, were reversed by amentoflavone treatment in a dose-dependent manner in MCF10DCIS.com cells. Congruent with the protein level, the activity of MMP-9 was significantly suppressed by amentoflavone treatment. Additionally, we found that amentoflavone dampened Gli1-dependent noncanonical hedgehog signaling, which is a key factor in the regulation of migration and invasion in TNFalpha-induced human breast cancer cells. Further study elucidated that TNFalpha enhanced Gli1 through the activation of the AKT/mTOR/S6K1 cascade, whereas it receded after amentoflavone treatment in human breast cancer cells. In summary, amentoflavone abrogated Gli1 activation in TNFalpha-induced mammary tumor cells, resulting in a decrease of invasiveness in human breast cancer cells via mediating AKT/mTOR/S6K1 signaling. Amentoflavone should be considered as a potent food ingredient for the retardation of mammary tumorigenesis.
- Journal
- Food Funct
- Publish Year
- 2021
- Experiment Subject
- human; mcf10dcis.com; mcf10dcis.com cells; mda-mb-231 cells; tnfalpha-induced breast cancer cells; tnfalpha-induced mammary tumor cells
- Experiment Type
- Cell Experiment
- Phenotype Related
- Carcinomas; Inflammatory Cytokine Tumor; Breast Cancer; Tnfalpha-induced Mammary Tumor
- Paper Title Cn
- Paper Title En
- Amentoflavone inhibits tumor necrosis factor-α-induced migration and invasion through AKT/mTOR/S6k1/hedgehog signaling in human breast cancer
- Bilingual Status
- semi_complete