ReferenceID 4465
Aloperine inhibits the progression of non-small-cell lung cancer through the PI3K/Akt signaling pathway
Cancer Cell Int
BACKGROUND: Lung cancer has become the leading cause of cancer-related death worldwide and non-small-cell lung cancer (NSCLC) accounts for approximately 85% of cases. Aloperine (ALO), an alkaloid active natural component
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- Reference Id
- 4465
- Evidence Id
- 21055
- Core Evidence Id
- 21055
- Source Reference Id
- 2217
- Herb2 Reference Id
- HBREF003014
- Subject Paper Key
- HBIN015319_34895234
- Pubmed Id
- 34895234
- Doi
- 10.1186/s12935-021-02361-5
- Paper Title
- Aloperine inhibits the progression of non-small-cell lung cancer through the PI3K/Akt signaling pathway
- Paper Abstract
- BACKGROUND: Lung cancer has become the leading cause of cancer-related death worldwide and non-small-cell lung cancer (NSCLC) accounts for approximately 85% of cases. Aloperine (ALO), an alkaloid active natural component from S. alopecuroide, has been found to exhibit anti-inflammatory, anti-tumor and anti-viral activity. However, Whether ALO exerts anti-tumor function on NSCLC remains poorly understood, and the underlying mechanisms remain unknown. METHODS: The CCK-8, colony formation, cell apoptosis with flow cytometry, wound healing and transwell cell invasion assays, were used to analyze the tumor progression of H1299 and A549 cells treated with ALO in vitro, and the xenograft model was constructed to assess the effect of ALO in vivo. The expression of protein was detected by Western blotting. RESULTS: ALO suppressed the cell proliferation, self-renewal, migration and invasion, induced apoptosis in A549 and H1299 cell. Furthermore, ALO significantly enhanced the level of cytochrome c in cytosol, and resulted in the dramatical increased levels of the cleaved caspase-3, caspased-9 and PARP. ALO also inhibited the expression of MMP-2 and MMP-9. Additionally, ALO also reduced p-AKT and p-mTOR to attenuate the PI3K/AKT signaling pathway. CONCLUSION: This study unveils a rationale for ALO through PI3K/Akt signaling pathway affecting the cell progression such as cell growth, apoptosis and invasion, and ALO acts as a potential chemotherapeutic agent for NSCLC.
- Journal
- Cancer Cell Int
- Publish Year
- 2021
- Experiment Subject
- a549 cells; h1299 cell
- Experiment Type
- Cell Experiment
- Phenotype Related
- Lung Cancer; Cancer-related Death; Tumor; Non-small-cell Lung Cancer
- Paper Title Cn
- Paper Title En
- Aloperine inhibits the progression of non-small-cell lung cancer through the PI3K/Akt signaling pathway
- Bilingual Status
- semi_complete