ReferenceID 4432

Antitumor effect of acanthoic acid against primary effusion lymphoma via inhibition of c-FLIP

Phytother Res

Acanthoic acid (AA) is an active substance that is extracted from Croton oblongifolius Roxb., a traditional plant in Thailand. The antiinflammatory effect of AA on NF-kappaB pathway has been exclusively reported, however

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Reference Id
4432
Evidence Id
21022
Core Evidence Id
21022
Source Reference Id
2145
Herb2 Reference Id
HBREF002942
Subject Paper Key
HBIN014341_34779075
Pubmed Id
34779075
Doi
10.1002/ptr.7322
Paper Title
Antitumor effect of acanthoic acid against primary effusion lymphoma via inhibition of c-FLIP
Paper Abstract
Acanthoic acid (AA) is an active substance that is extracted from Croton oblongifolius Roxb., a traditional plant in Thailand. The antiinflammatory effect of AA on NF-kappaB pathway has been exclusively reported, however, its anticancer effect is still lacking. PEL is a B cell lymphoma that is mostly found in HIV patients. The prognosis and progression of PEL patients are terribly poor with a median survival time less than 6 months, so the new effective treatment is urgently needed. In this study, we found that AA effectively inhibited PEL cell proliferation with IC50s at 120-130 muM in well-representative cells, while the IC50s of AA in PBMC were higher (>200 muM). AA increased percentages of Annexin V/PI positive cells, whereas adding of caspase inhibitor (Q-VD-OPh) prevented AA-induced cell death. The antiapoptotic protein, c-FLIP, was downregulated by AA which leading to the activation of caspase-8 and -3. Combination of AA and TRAIL dramatically enhanced apoptotic cell death. In PEL xenograft model, AA at the dose of 250 mg/kg effectively inhibited PEL tumor growth without detectable toxicities assessed by mice weight and appearance.
Journal
Phytother Res
Publish Year
2021
Experiment Subject
mouse; patient; annexin v/pi positive cells; pel cell; pel xenograft model
Experiment Type
Cell Experiment
Phenotype Related
Tumor; B Cell Lymphoma
Paper Title Cn
Paper Title En
Antitumor effect of acanthoic acid against primary effusion lymphoma via inhibition of c-FLIP
Bilingual Status
semi_complete