ReferenceID 4427
Acacetin exerts antioxidant potential against atherosclerosis through Nrf2 pathway in apoE-/- Mice
J Cell Mol Med
Oxidative stress has a considerable influence on endothelial cell dysfunction and atherosclerosis. Acacetin, an anti-inflammatory and antiarrhythmic, is frequently used in the treatment of myocarditis, albeit its role in
Relationship Network
Interactive first-hop connections across herbs, ingredients, formulas, targets, diseases, symptoms, syndromes, evidence, and monographs.
Click a node to open it in a new tab
Ingredient: 1Reference: 1Links: 1
Arranging relationship network...
Record Fields
Scalar fields from the final reference record.
- Reference Id
- 4427
- Evidence Id
- 21017
- Core Evidence Id
- 21017
- Source Reference Id
- 2135
- Herb2 Reference Id
- HBREF002932
- Subject Paper Key
- HBIN014294_33241629
- Pubmed Id
- 33241629
- Doi
- 10.1111/jcmm.16106
- Paper Title
- Acacetin exerts antioxidant potential against atherosclerosis through Nrf2 pathway in apoE-/- Mice
- Paper Abstract
- Oxidative stress has a considerable influence on endothelial cell dysfunction and atherosclerosis. Acacetin, an anti-inflammatory and antiarrhythmic, is frequently used in the treatment of myocarditis, albeit its role in managing atherosclerosis is currently unclear. Thus, we evaluated the regulatory effects of acacetin in maintaining endothelial cell function and further investigated whether the flavonoid could attenuate atherosclerosis in apolipoprotein E deficiency (apoE-/- ) mice. Different concentrations of acacetin were tested on EA.hy926 cells, either induced or non-induced by human oxidized low-density lipoprotein (oxLDL), to clarify its influence on cell viability, cellular reactive oxidative stress (ROS) level, apoptotic ratios and other regulatory effects. In vivo, apoE-/- mice were fed either a Western diet or a chow diet. Acacetin pro-drug (15 mg/kg) was injected subcutaneously two times a day for 12 weeks. The effects of acacetin on the atherosclerotic process, plasma inflammatory factors and lipid metabolism were also investigated. Acacetin significantly increased EA.hy926 cell viability by reducing the ratios of apoptotic and necrotic cells at 3 mumol/L. Moreover, 3 mumol/L acacetin clearly decreased ROS levels and enhanced reductase protein expression through MsrA and Nrf2 pathway through phosphorylation of Nrf2 and degradation of Keap1. In vivo, acacetin treatment remarkably attenuated atherosclerosis by increasing reductase levels in circulation and aortic roots, decreasing plasma inflammatory factor levels as well as accelerating lipid metabolism in Western diet-fed apoE-/- mice. Our findings demonstrate the anti-oxidative and anti-atherosclerotic effects of acacetin, in turn suggesting its potential therapeutic value in atherosclerotic-related cardiovascular diseases (CVD).
- Journal
- J Cell Mol Med
- Publish Year
- 2021
- Experiment Subject
- mouse; human; ea.hy926 cell; ea.hy926 cells
- Experiment Type
- Animal & Cell Experiment
- Phenotype Related
- Apolipoprotein E Deficiency; Atherosclerosis; Myocarditis; Atherosclerotic-related Cardiovascular Diseases; Endothelial Cell Dysfunction
- Paper Title Cn
- Paper Title En
- Acacetin exerts antioxidant potential against atherosclerosis through Nrf2 pathway in apoE-/- Mice
- Bilingual Status
- semi_complete