ReferenceID 4366
Novel antiadipogenic effect of menadione in 3T3-L1 cells
Chem Biol Interact
Inhibition of adipocyte differentiation can be used as a strategy for preventing adipose tissue expansion and, consequently, for obesity management. Since reactive oxygen species (ROS) have emerged as key modulators of a
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Record Fields
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- Reference Id
- 4366
- Evidence Id
- 20956
- Core Evidence Id
- 20956
- Source Reference Id
- 1993
- Herb2 Reference Id
- HBREF002790
- Subject Paper Key
- HBIN005945_33945810
- Pubmed Id
- 33945810
- Doi
- 10.1016/j.cbi.2021.109491
- Paper Title
- Novel antiadipogenic effect of menadione in 3T3-L1 cells
- Paper Abstract
- Inhibition of adipocyte differentiation can be used as a strategy for preventing adipose tissue expansion and, consequently, for obesity management. Since reactive oxygen species (ROS) have emerged as key modulators of adipogenesis, the effect of menadione (a synthetic form of vitamin K known to induce the increase of intracellular ROS) on 3T3-L1 preadipocyte differentiation was studied. Menadione (15 muM) increased ROS and lipid peroxidation, generating mild oxidative stress without affecting cell viability. Menadione drastically inhibited adipogenesis, accompanied by decreased intracellular lipid accumulation and diminished expression of the lipo/adipogenic markers peroxisome proliferator-activated receptor (PPAR)gamma, fatty acid synthase (FAS), CCAAT/enhancer-binding protein (C/EBP) alpha, fatty acid binding protein (FABP) 4, and perilipin. Menadione treatment also increased lipolysis, as indicated by augmented glycerol release and reinforced by the increased expression of hormone-sensitive lipase (HSL). Additionally, menadione increased the inhibitory phosphorylation of acetyl-CoA-carboxylase (ACC), which results in the inhibition of fatty acid synthesis. As a consequence, triglyceride content was decreased. Menadione also inhibited the phosphatidylinositol 3-kinase (PI3K)/Akt pathway. Further, treatment with increased concentration of insulin, a potent physiological activator of the PI3K/Akt pathway, rescued the normal level of expression of PPARgamma, the master regulator of adipogenesis, and overcame the restraining effect of menadione on the differentiation capacity of 3T3-L1 preadipocytes. Our study reveals novel antiadipogenic action for menadione, which is, at least in part, mediated by the PI3K/Akt pathway signaling and raises its potential as a therapeutic agent in the treatment or prevention of adiposity.
- Journal
- Chem Biol Interact
- Publish Year
- 2021
- Experiment Subject
- Experiment Type
- Cell Experiment
- Phenotype Related
- Obesity
- Paper Title Cn
- Paper Title En
- Novel antiadipogenic effect of menadione in 3T3-L1 cells
- Bilingual Status
- semi_complete