ReferenceID 4341
Natural product corynoline suppresses melanoma cell growth through inducing oxidative stress
Phytother Res
Natural product corynoline is a unique isoquinoline alkaloid extracted from traditional Chinese medicine Corydalis bungeana Turcz, whereas its anticancer properties have not been investigated. In this study, we found tha
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Record Fields
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- Reference Id
- 4341
- Evidence Id
- 20931
- Core Evidence Id
- 20931
- Source Reference Id
- 1949
- Herb2 Reference Id
- HBREF002746
- Subject Paper Key
- HBIN002081_32430958
- Pubmed Id
- 32430958
- Doi
- 10.1002/ptr.6719
- Paper Title
- Natural product corynoline suppresses melanoma cell growth through inducing oxidative stress
- Paper Abstract
- Natural product corynoline is a unique isoquinoline alkaloid extracted from traditional Chinese medicine Corydalis bungeana Turcz, whereas its anticancer properties have not been investigated. In this study, we found that corynoline potently impairs the growth of melanoma cells, B16F10, and A375 in a concentration-dependent manner. Treatment of melanoma cells with corynoline results in G2 cell arrest accompanied by reduced cdc2 activation. Furthermore, corynoline triggers apoptosis of melanoma cells, which is associated with increased expression of Bax and cleaved caspase-3. Mechanistic study indicates that corynoline strongly induces reactive oxygen species (ROS) generation and subsequent DNA damage as evidenced by gamma-H2AX accumulation. Notably, the effect of corynoline on melanoma cell cycle and apoptosis is abolished by a ROS scavenger N-acetyl cysteine (NAC), indicating a ROS-dependent mechanism. Finally, corynoline significantly inhibits in vivo B16F10 melanoma tumor growth accompanied by reduced expression of Ki-67 in tumor tissue. Taken together, our data suggest that corynoline suppresses melanoma cell growth in vitro and in vivo by inducing oxidative stress and represents a potential therapeutic agent for melanoma patients.
- Journal
- Phytother Res
- Publish Year
- 2020
- Experiment Subject
- patient
- Experiment Type
- Cell Experiment
- Phenotype Related
- Melanoma; Tumor; B16f10 Melanoma Tumor
- Paper Title Cn
- Paper Title En
- Natural product corynoline suppresses melanoma cell growth through inducing oxidative stress
- Bilingual Status
- semi_complete