ReferenceID 4269
Saffron inhibits the proliferation of hepatocellular carcinoma via inducing cell apoptosis
Panminerva Med
BACKGROUND: Liver cancer remains the third leading cause of cancer-related mortality worldwide. The aim of this study was to explore the effect of saffron on liver cancer cell line QGY-7703 and the underlying molecular m
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- Reference Id
- 4269
- Evidence Id
- 20859
- Core Evidence Id
- 20859
- Source Reference Id
- 1814
- Herb2 Reference Id
- HBREF002611
- Subject Paper Key
- HERB005826_30657284
- Pubmed Id
- 30657284
- Doi
- 10.23736/S0031-0808.18.03561-9
- Paper Title
- Saffron inhibits the proliferation of hepatocellular carcinoma via inducing cell apoptosis
- Paper Abstract
- BACKGROUND: Liver cancer remains the third leading cause of cancer-related mortality worldwide. The aim of this study was to explore the effect of saffron on liver cancer cell line QGY-7703 and the underlying molecular mechanism. METHODS: Cell growth was detected by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay and cell cycle was assessed by flow cytometry. Besides, cell apoptosis was analyzed by Annexin V/PI (Propidium Iodide) staining, and the senescent cells morphology staining of beta-galactosidase was evaluated by microscopy. In addition, ELISA (enzyme-linked immunosorbent assay) Kit was used to assess the activity of telomerase. Moreover, reverse transcription-PCR (polymerase chain reaction) and Western blot analysis was applied to detect mRNA and protein expression levels, respectively. RESULTS: Saffron treatment in QGY-7703 cells could significantly inhibit cell growth, arrest cell cycle in the G0/G1 phase, and induce cell apoptosis. Besides, the treatment of saffron could obviously decrease telomerase activity and hTERT level in QGY-7703 cells. In addition, enhanced Bax/Bcl-2 ratio and increased expression of P21 were found in saffron-treated cells. Moreover, we found that the number of senescent cells increased dramatically and the morphology of cells changed obviously after saffron treatment. CONCLUSIONS: Saffron administration may provide some experimental evidence for the inhibitory effect of saffron on the proliferation of QGY-7703 cells, suggesting that saffron may have potential utility for the treatment of liver cancer.
- Journal
- Panminerva Med
- Publish Year
- 2020
- Experiment Subject
- Experiment Type
- Cell Experiment
- Phenotype Related
- Liver Cancer
- Paper Title Cn
- Paper Title En
- Saffron inhibits the proliferation of hepatocellular carcinoma via inducing cell apoptosis
- Bilingual Status
- semi_complete