ReferenceID 4154
Structural characteristics and immunostimulatory activities of a new polysaccharide from Dendrobium fimbriatum Hook
Food Funct
A new polysaccharide (cDFP-W1) with high immunostimulatory activities was isolated from the stems of Dendrobium fimbriatum Hook. The analysis of the physicochemical properties showed that cDFP-W1 consisted of mannose and
Relationship Network
Interactive first-hop connections across herbs, ingredients, formulas, targets, diseases, symptoms, syndromes, evidence, and monographs.
Click a node to open it in a new tab
Herb: 1Reference: 1Links: 1
Arranging relationship network...
Record Fields
Scalar fields from the final reference record.
- Reference Id
- 4154
- Evidence Id
- 20744
- Core Evidence Id
- 20744
- Source Reference Id
- 1581
- Herb2 Reference Id
- HBREF002378
- Subject Paper Key
- HERB003464_33710189
- Pubmed Id
- 33710189
- Doi
- 10.1039/d0fo03336g
- Paper Title
- Structural characteristics and immunostimulatory activities of a new polysaccharide from Dendrobium fimbriatum Hook
- Paper Abstract
- A new polysaccharide (cDFP-W1) with high immunostimulatory activities was isolated from the stems of Dendrobium fimbriatum Hook. The analysis of the physicochemical properties showed that cDFP-W1 consisted of mannose and glucose in a molar ratio of 1 : 3.84, and its relative molecular weight was 4.0 x 104 Da. Structural analysis implied that the linear backbone of cDFP-W1 was composed of alpha-1,4-d-Glcp, beta-1,4-d-Manp, 3-O-acetyl-alpha-1,4-d-Glcp and alpha-1,4,6-d-Glcp, and its branches were the terminal beta-d-Manp that was attached to the C-6 position of alpha-1,4,6-d-Glcp. An in vivo immunostimulatory assay exhibited that cDFP-W1 at 200 mg kg-1 could significantly increase the proportions of CD4+ T-cell subpopulations, B cells, natural killer cells and dendritic cells, decrease the proportion of CD8+ T-cell subpopulations, and upregulate the percentage of activated macrophages (p < 0.01) in the spleen of mice. An in vitro immunostimulatory assay revealed that cDFP-W1 could effectively promote the proliferation of spleen lymphocytes, enhance the proliferation and phagocytosis of macrophage RAW264.7 cells, and stimulate the mRNA expression and extracellular release of NO, TNF-alpha and IL-1beta of RAW264.7 cells. The western blot experiment suggested that the immunostimulatory activities of cDFP-W1 were closely related to the activation of MAPKs, NF-kappaB and PI3K/Akt signaling pathways.
- Journal
- Food Funct
- Publish Year
- 2021
- Experiment Subject
- mouse; macrophage raw264.7 cells; raw264.7 cells
- Experiment Type
- Cell Experiment
- Phenotype Related
- Paper Title Cn
- Paper Title En
- Structural characteristics and immunostimulatory activities of a new polysaccharide from Dendrobium fimbriatum Hook
- Bilingual Status
- semi_complete