ReferenceID 4138
Sambucus williamsii Hance maintains bone homeostasis in hyperglycemia-induced osteopenia by reversing oxidative stress via cGMP/PKG signal transduction
Phytomedicine
Background: Sambucus williamsii Hance (SWH) has effectively been adopted to treat joint and bone disorders. Diabetes-induced osteopenia (DOP) is caused primarily by impaired bone formation as a result of hyperglycemia. W
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Record Fields
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- Reference Id
- 4138
- Evidence Id
- 20728
- Core Evidence Id
- 20728
- Source Reference Id
- 1548
- Herb2 Reference Id
- HBREF002345
- Subject Paper Key
- HERB002877_36610352
- Pubmed Id
- 36610352
- Doi
- 10.1016/j.phymed.2022.154607
- Paper Title
- Sambucus williamsii Hance maintains bone homeostasis in hyperglycemia-induced osteopenia by reversing oxidative stress via cGMP/PKG signal transduction
- Paper Abstract
- Background: Sambucus williamsii Hance (SWH) has effectively been adopted to treat joint and bone disorders. Diabetes-induced osteopenia (DOP) is caused primarily by impaired bone formation as a result of hyperglycemia. We had previously demonstrated that SWH extract accelerated fracture healing and promoted osteoblastic MC3T3-E1 cell proliferation and osteogenic differentiation. This study assessed the impacts of SWH extract on diabetes-induced bone loss and explored the mechanisms underlying its osteoprotective effects. Methods: This work employed MC3T3-E1 cell line for evaluating how SWH extract affected osteogenesis, oxidative stress (OS), and the underlying mechanism in vitro. Streptozotocin-induced osteopenia mouse model was applied with the purpose of assessing SWH extract's osteoprotection on bone homeostasis in vivo. Results: The increased OS of MC3T3-E1 cells exposed to high glucose (HG) was largely because of the upregulation of pro-oxidant genes and the downregulation of antioxidant genes, whereas SWH extract reduced the OS by modulating NADPH oxidase-4 and thioredoxin-related genes by activating cyclic guanosine monophosphate (cGMP) production and increasing the level of cGMP-mediated protein kinase G type-2 (PKG2). The oral administration of SWH extract maintained bone homeostasis in type 1 diabetes mellitus (T1DM) mice by enhancing osteogenesis while decreasing OS. In bones from hyperglycemia-induced osteopenia mice and HG-treated MC3T3-E1 cells, the SWH extract achieved the osteoprotective effects through activating the cGMP/PKG2 signaling pathway, upregulating the level of antioxidant genes, as well as downregulating the level of pro-oxidant genes. Conclusion: SWH extract exerts osteoprotective effects on hyperglycemia-induced osteopenia by reversing OS via cGMP/PKG signal transduction and is a potential therapy for DOP.
- Journal
- Phytomedicine
- Publish Year
- 2023
- Experiment Subject
- mouse; hg-treated mc3t3-e1 cells; mc3t3-e1 cell line; mc3t3-e1 cells; osteoblastic mc3t3-e1 cell
- Experiment Type
- Cell Experiment
- Phenotype Related
- Hyperglycemia; Type 1 Diabetes Mellitus; Streptozotocin-induced Osteopenia; Joint And Bone Disorders; Hyperglycemia-induced Osteopenia; Diabetes-induced Osteopenia
- Paper Title Cn
- Paper Title En
- Sambucus williamsii Hance maintains bone homeostasis in hyperglycemia-induced osteopenia by reversing oxidative stress via cGMP/PKG signal transduction
- Bilingual Status
- semi_complete