ReferenceID 4111

Succinum extracts inhibit microglial-derived neuroinflammation and depressive-like behaviors

Front Pharmacol

Microglia are emerging as important targets for the treatment of neuropsychiatric disorders. The phagocytic microglial phenotype and the resulting neuroinflammation lead to synaptic loss and neuronal cell death. To explo

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Reference Id
4111
Evidence Id
20701
Core Evidence Id
20701
Source Reference Id
1499
Herb2 Reference Id
HBREF002296
Subject Paper Key
HERB002385_36052132
Pubmed Id
36052132
Doi
10.3389/fphar.2022.991243
Paper Title
Succinum extracts inhibit microglial-derived neuroinflammation and depressive-like behaviors
Paper Abstract
Microglia are emerging as important targets for the treatment of neuropsychiatric disorders. The phagocytic microglial phenotype and the resulting neuroinflammation lead to synaptic loss and neuronal cell death. To explore potential candidates that inhibit microglial hyperactivation, we first investigated ten candidate extracts of traditional Chinese medicine (TCM) using lipopolysaccharide (LPS)-stimulated BV2 microglial cells. Among the candidates, Pinus spp. succinum extract (PSE) was superior; thus, we further investigated its pharmacological activity and underlying mechanisms both in vitro and in vivo . Pretreatment with PSE (10, 20, and 40 μg/ml) attenuated the increases in inflammatory factors (nitric oxide and tumor necrosis factor-α), translocation of nuclear factor-kappa B (NF-κB), and phenotypic transformations (phagocytic and migratory) in a dose-dependent manner. These inhibitory effects of PSE on microglia were supported by its regulatory effects on the CX 3 C chemokine receptor 1 (CX 3 CR1)/nuclear factor erythroid-2-related factor 2 (Nrf2) pathway. In particular, intragastric administration of PSE (100 mg/kg) considerably improved sickness, anxiety, and depressive-like behaviors in mice subjected to chronic restraint stress (CRS). Our results suggest that PSE has strong antineuroinflammatory and antidepressant properties, and the underlying mechanisms may involve not only the regulation of NF-κB translocation but also the normalization of the CX 3 CR1/Nrf2 pathway.
Journal
Front Pharmacol
Publish Year
2022
Experiment Subject
mouse; lipopolysaccharide (lps)-stimulated bv2 microglial cells
Experiment Type
Cell Experiment
Phenotype Related
Neuropsychiatric Disorders; Neuronal Cell Death; Chronic Restraint Stress; Anxiety
Paper Title Cn
Paper Title En
Succinum extracts inhibit microglial-derived neuroinflammation and depressive-like behaviors
Bilingual Status
semi_complete