ReferenceID 407
Wogonoside induces growth inhibition and cell cycle arrest via promoting the expression and binding activity of GATA-1 in chronic myelogenous leukemia cells
Arch Toxicol
GATA-1, a zinc finger transcription factor, has been demonstrated to play a key role in the progression of leukemia. In this study, we investigate the effects of wogonoside, a naturally bioactive flavonoid derived from S
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Record Fields
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- Reference Id
- 407
- Evidence Id
- 16997
- Core Evidence Id
- 16997
- Source Reference Id
- 782
- Herb2 Reference Id
- HBREF001347
- Subject Paper Key
- HBIN048376_26104856
- Pubmed Id
- 26104856
- Doi
- 10.1007/s00204-015-1552-3
- Paper Title
- Wogonoside induces growth inhibition and cell cycle arrest via promoting the expression and binding activity of GATA-1 in chronic myelogenous leukemia cells
- Paper Abstract
- GATA-1, a zinc finger transcription factor, has been demonstrated to play a key role in the progression of leukemia. In this study, we investigate the effects of wogonoside, a naturally bioactive flavonoid derived from Scutellaria baicalensis Georgi, on cell growth and cell cycle in chronic myeloid leukemia (CML) cells, and uncover its underlying mechanisms. The experimental design comprised CML cell lines K562, imatinib-resistant K562 (K562r) cells, and primary CML cells, treated in vitro or in vivo, respectively, with wogonoside; growth and cell cycle were then evaluated. We found that wogonoside could induce growth inhibition and G0/G1 cell cycle arrest in both normal and K562r cells. Wogonoside promotes the expression of GATA-1 and facilitates the binding to methyl ethyl ketone (MEK) and p21 promoter, thus inhibiting MEK/extracellular signal-regulated kinase signaling and cell cycle checkpoint proteins, including CDK2, CDK4, cyclin A, and cyclin D1, and increasing p21 expression. Furthermore, in vivo studies showed that administration of wogonoside decreased CML cells and prolonged survival in NOD/SCID mice with CML cell xenografts. In conclusion, these results clearly revealed the inhibitory effect of wogonoside on the growth in CML cells and suggested that wogonoside may act as a promising drug for the treatment of imatinib-resistant CML.
- Journal
- Arch Toxicol
- Publish Year
- 2016
- Experiment Subject
- chronic myeloid leukemia (cml) cells,nod/scid mice
- Experiment Type
- Animal & Cell Experiment
- Phenotype Related
- Myelogenous Leukemia; Leukemia
- Paper Title Cn
- Paper Title En
- Wogonoside induces growth inhibition and cell cycle arrest via promoting the expression and binding activity of GATA-1 in chronic myelogenous leukemia cells
- Bilingual Status
- semi_complete