ReferenceID 4046
Petasites japonicus extract exerts anti-malarial effects by inhibiting platelet activation
Phytomedicine
Background: New antimalarial agents are needed to combat emerging resistance to the currently available drugs. In the pathology of cerebral malaria, platelets play a central role by binding infected and uninfected red ce
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- Reference Id
- 4046
- Evidence Id
- 20636
- Core Evidence Id
- 20636
- Source Reference Id
- 1376
- Herb2 Reference Id
- HBREF002173
- Subject Paper Key
- HERB001700_35598522
- Pubmed Id
- 35598522
- Doi
- 10.1016/j.phymed.2022.154167
- Paper Title
- Petasites japonicus extract exerts anti-malarial effects by inhibiting platelet activation
- Paper Abstract
- Background: New antimalarial agents are needed to combat emerging resistance to the currently available drugs. In the pathology of cerebral malaria, platelets play a central role by binding infected and uninfected red cells and the endothelium. Since Petasites japonicus extract was reported as an effective inhibitor of platelet activation, we examined the antimalarial activities of the P. japonicus extract. Purpose: This study aimed to evaluate the impact of P. japonicus extract prepared from whole plants on malarial infection. Methods: The P. japonicus extract were characterized by high-performance liquid chromatography (HPLC) profiling. Antimalarial activity of the P. japonicus ethanolic extract was evaluated in vitro using chloroquine-sensitive (3D7) and chloroquine-resistant (Dd2) P. berghei strains. Also, the in vivo activity of the extract was evaluated in P. berghei-infected mice via oral administration followed by a four-day suppressive test to measure the hematological parameters. In addition, platelet activation signaling induced by the P. japonicus extract in P. berghei infection was evaluated. Results: In HPLC study, catechin, rutin, liquiritin, 3,4-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid, and 4,5-di-O-caffeoylquinic acid were identified in P. japonicus extract. Exposure to the P. japonicus extract significantly inhibited both CQ-sensitive (3D7) and resistant (Dd2) strains of P. falciparum with IC 50 values of 8.48 ± 1.70 and 7.83 ± 6.44 μg/ml, respectively. Administration of the P. japonicus extract also resulted in potent antimalarial activities in P. berghei-infected mice with no associated toxicity. The treatment also improved the hematologic parameters. In addition, the survived mice from P. berghei infection exhibited the inhibition of collagen-induced platelet aggregation by attenuated glycoprotein VI (GPVI) downstream signaling. Conclusion: P. japonicus extracts promote antimalarial effects both in vitro and in vivo. In addition, the effects appear to be induced by the inhibition of collagen-induced platelet activation related to attenuated GPVI downstream signaling. Further studies to identify and characterize the antimalarial compounds in P. japonicus will promote the development of new drugs.
- Journal
- Phytomedicine
- Publish Year
- 2022
- Experiment Subject
- mouse
- Experiment Type
- Animal Experiment
- Phenotype Related
- Cerebral Malaria
- Paper Title Cn
- Paper Title En
- Petasites japonicus extract exerts anti-malarial effects by inhibiting platelet activation
- Bilingual Status
- semi_complete