ReferenceID 4045
Immunostimulatory Potential of Extracellular Vesicles Isolated from an Edible Plant, Petasites japonicus, via the Induction of Murine Dendritic Cell Maturation
Int J Mol Sci
Extracellular vesicles (EVs) have recently been isolated from different plants. Plant-derived EVs have been proposed as potent therapeutics and drug-delivery nanoplatforms for delivering biomolecules, including proteins,
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Record Fields
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- Reference Id
- 4045
- Evidence Id
- 20635
- Core Evidence Id
- 20635
- Source Reference Id
- 1375
- Herb2 Reference Id
- HBREF002172
- Subject Paper Key
- HERB001700_34638974
- Pubmed Id
- 34638974
- Doi
- 10.3390/ijms221910634
- Paper Title
- Immunostimulatory Potential of Extracellular Vesicles Isolated from an Edible Plant, Petasites japonicus, via the Induction of Murine Dendritic Cell Maturation
- Paper Abstract
- Extracellular vesicles (EVs) have recently been isolated from different plants. Plant-derived EVs have been proposed as potent therapeutics and drug-delivery nanoplatforms for delivering biomolecules, including proteins, RNAs, DNAs, and lipids. Herein, Petasites japonicus-derived EVs (PJ-EVs) were isolated through a series of centrifugation steps and characterized using dynamic light scattering and transmission electron microscopy. Immunomodulatory effects of PJ-EVs were assessed using dendritic cells (DCs). PJ-EVs exhibited a spherical morphology with an average size of 122.6 nm. They induced the maturation of DCs via an increase in the expression of surface molecules (CD80, CD86, MHC-I, and MHC-II), production of Th1-polarizing cytokines (TNF-alpha and IL-12p70), and antigen-presenting ability; however, they reduced the antigen-uptake ability. Furthermore, maturation of DCs induced by PJ-EVs was dependent on the activation and phosphorylation of MAPK and NF-kappaB signal pathways. Notably, PJ-EV-treated DCs strongly induced the proliferation and differentiation of naive T cells toward Th1-type T cells and cytotoxic CD8+ T cells along with robust secretion of IFN-gamma and IL-2. In conclusion, our study indicates that PJ-EVs can be potent immunostimulatory candidates with an ability of strongly inducing the maturation of DCs.
- Journal
- Int J Mol Sci
- Publish Year
- 2021
- Experiment Subject
- pj-ev-treated dcs
- Experiment Type
- Cell Experiment
- Phenotype Related
- Paper Title Cn
- Paper Title En
- Immunostimulatory Potential of Extracellular Vesicles Isolated from an Edible Plant, Petasites japonicus, via the Induction of Murine Dendritic Cell Maturation
- Bilingual Status
- semi_complete