ReferenceID 4038
Apoptotic-Induced Effects of Acacia Catechu Willd. Extract in Human Colon Cancer Cells
Int J Mol Sci
The research for innovative treatments against colon adenocarcinomas is still a great challenge. Acacia catechu Willd. heartwood extract (AC) has health-promoting qualities, especially at the gastrointestinal level. This
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Record Fields
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- Reference Id
- 4038
- Evidence Id
- 20628
- Core Evidence Id
- 20628
- Source Reference Id
- 1357
- Herb2 Reference Id
- HBREF002154
- Subject Paper Key
- HERB001583_32204339
- Pubmed Id
- 32204339
- Doi
- 10.3390/ijms21062102
- Paper Title
- Apoptotic-Induced Effects of Acacia Catechu Willd. Extract in Human Colon Cancer Cells
- Paper Abstract
- The research for innovative treatments against colon adenocarcinomas is still a great challenge. Acacia catechu Willd. heartwood extract (AC) has health-promoting qualities, especially at the gastrointestinal level. This study characterized AC for its catechins content and investigated the apoptosis-enhancing effect in human colorectal adenocarcinoma HT-29 cells, along with its ability to spare healthy tissue. MTT assay was used to describe the time course, concentration dependence and reversibility of AC-mediated cytotoxicity. Cell cycle analysis and AV-PI and DAPI-staining were performed to evaluate apoptosis, together with ROS formation, mitochondrial membrane potential (MMP) changes and caspase activities. Rat ileum and colon rings were tested for their viability and functionality to explore AC effects on healthy tissue. Quantitative analysis highlighted that AC was rich in (+-)-catechin (31.5 +- 0.82 mg/g) and (-)-epicatechin (12.5 +- 0.42 mg/g). AC irreversibly decreased cell viability in a concentration-dependent, but not time-dependent fashion. Cytotoxicity was accompanied by increases in apoptotic cells and ROS, a reduction in MMP and increases in caspase-9 and 3 activities. AC did not affect rat ileum and colon rings' viability and functionality, suggesting a safe profile toward healthy tissue. The present findings outline the potential of AC for colon cancer treatment.
- Journal
- Int J Mol Sci
- Publish Year
- 2020
- Experiment Subject
- rat; human; human colorectal adenocarcinoma ht-29 cells
- Experiment Type
- Cell Experiment
- Phenotype Related
- Colorectal Adenocarcinoma; Colon Adenocarcinomas; Colon Cancer
- Paper Title Cn
- Paper Title En
- Apoptotic-Induced Effects of Acacia Catechu Willd. Extract in Human Colon Cancer Cells
- Bilingual Status
- semi_complete